Genetic Diversity and Population Structure of Local Tea Landraces in Sichuan and Chongqing Revealed by EST-SSR Markers

YAO Ming-zhe; MA Chun-lei; JIN Ji-qiang; MA Jian-qiang; CHEN Liang

doi:10.13305/j.cnki.jts.2012.05.011

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Journal of Tea Science ›› 2012, Vol. 32 ›› Issue (5) : 419-425. DOI: 10.13305/j.cnki.jts.2012.05.011

Genetic Diversity and Population Structure of Local Tea Landraces in Sichuan and Chongqing Revealed by EST-SSR Markers

YAO Ming-zhe, MA Chun-lei, JIN Ji-qiang, MA Jian-qiang, CHEN Liang^*

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Abstract

Ninety local tea landraces collected from Sichuan and Chongqing provinces were used to analyze genetic diversity and population structure based on 84 EST-SSR markers. A total of 275 alleles were amplified, with an average of 3.3 alleles and 0.33 of heterozygosity per locus. The mean gene diversity (H) and polymorphic information content (PIC) were estimated to be 0.462 and 0.407, respectively, among 90 tested accessions. The level of genetic diversity among local tea landraces in Chongqing was higher than that in Sichuan. And comparing to tea landraces from north part (Lat.≥N32°), the genetic diversity was significant higher in the southern part (Lat.

Key words

Camellia sinensis / EST-SSR / genetic diversity / landrace / population structure

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YAO Ming-zhe, MA Chun-lei, JIN Ji-qiang, MA Jian-qiang, CHEN Liang. Genetic Diversity and Population Structure of Local Tea Landraces in Sichuan and Chongqing Revealed by EST-SSR Markers[J]. Journal of Tea Science. 2012, 32(5): 419-425 https://doi.org/10.13305/j.cnki.jts.2012.05.011

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