Complete Chloroplast Genome of Camellia sinensis cv. Xinyang 10 and Its Phylogenetic Evolution

YAN Minghui; LIU Ke; WANG Man; LYU Ying; ZHANG Qian

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Journal of Tea Science ›› 2021, Vol. 41 ›› Issue (6) : 777-788.

Research Paper

Complete Chloroplast Genome of Camellia sinensis cv. Xinyang 10 and Its Phylogenetic Evolution

YAN Minghui¹, LIU Ke², WANG Man², LYU Ying¹, ZHANG Qian¹

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Abstract

Camellia sinensis cv. Xinyang 10 is a national excellent cultivar suitable for producing Xinyangmaojian. However, its origin and evolutionary relationship with other tea cultivars are still unknown. This study obtained the complete chloroplast genome of C. sinensis cv. Xinyang 10 by using MGI2000 sequencing platform, and then analyzed the chloroplast genome structure. A chloroplast genome phylogenetic analysis of 46 species was also conducted to infer the position of C. sinensis cv. Xinyang 10. The results show that: (1) the chloroplast genome of Xinyang 10 is 157 041 bp in length, including a pair of inverted repeat regions (IR, 26 078 bp), a large single-copy region (LSC, 86 594 bp) and a small single-copy region (SSC, 18 291 bp). A total of 113 genes are annotated, including 79 protein-coding genes, 30 tRNA genes, and 4 rRNA genes. (2) 74 SSR loci were detected in the chloroplast genome of Xinyang 10, most of the SSRs were composed of A/T. (3) The cluster analysis using Bayesian method shows that Xinyang 10 had the closest genetic relationship with C. sinensis cv. Tieluohan. Both cultivars may share the same female parent as their chloroplast genomes were identical. Xinyang 10 also had a close relationship with two Korean tea (Chamnok and Sangmok), Baijiguan and C. sinensis var. Dehungensis. The results provided a basis for further research on the origin and evolution of tea and molecular breeding.

Key words

C. sinensis cv. Xinyang 10 / Camellia sinensis / chloroplast genome / phylogentic analysis

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YAN Minghui, LIU Ke, WANG Man, LYU Ying, ZHANG Qian. Complete Chloroplast Genome of Camellia sinensis cv. Xinyang 10 and Its Phylogenetic Evolution[J]. Journal of Tea Science. 2021, 41(6): 777-788

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