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Journal of Tea Science ›› 2023, Vol. 43 ›› Issue (2): 250-262.doi: 10.13305/j.cnki.jts.2023.02.004

• Research Paper • Previous Articles     Next Articles

Simultaneous Determination of 79 Pesticides Residues in Fresh Tea Leaves by Ultra-high Performance Liquid Chromatography-mass Spectrometry

PAN Yingjie1,2, MENG Xianghe1, QIAN Yuanfeng2, NING Jianmei2, WANG Yueyuan2, LIU Qiming2, TANG Shiqin2, XU Xiaoan2, YE Qin3,*   

  1. 1. College of Food Science and Technology, Zhejiang University of Technology, Hangzhou 310018, China;
    2. Agriculture and Rural Affairs Bureau, Songyang 323400, China;
    3. Department of Biological and Environmental Engineering, Zhejiang Shuren University, Hangzhou 310014, China
  • Received:2022-11-14 Revised:2023-02-10 Online:2023-04-15 Published:2023-05-05

Abstract: A method for simultaneous determination of 79 pesticides residues in fresh tea leaves by ultra-high performance liquid chromatography-mass spectrometry (UPLC-MS/MS) was establish in this study. The fresh tea leaves were ground by high-speed wall breaking and then extracted by acetonitrile solution with NaCl. The liquid supernatant was centrifuged and purified by solid phase extraction of anhydrous magnesium sulfate, C18, PSA and GCB. The scheduled multiple reaction monitoring mode (sMRM) was used for scanning and determination, and quantitative by external standard method. The results show that the 79 target compounds exhibited good linearity within the concentration range of 0.001~0.400 mg·L-1 with a correlation coefficient (R2) of 0.990 6 to 0.999 9. The average recoveries at 5 spiked levels (0.01, 0.03, 0.05, 0.10, 0.20 mg·kg-1) ranged from 70.7% to 119.0%, with the relative standard deviations (RSDs) ranging from 0.1% to 19.8%, and the limits of quantitation (LOQ) for the 79 pesticides ranging from 0.01 mg·kg-1 to 0.05 mg·kg-1. The proposed method is high sensitivity, good accuracy and quick, which is suitable for determination of 79 pesticides residues in fresh tea leaves.

Key words: fresh tea leaves, UPLC-MS/MS, pesticide residues, recovery

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