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Journal of Tea Science ›› 2022, Vol. 42 ›› Issue (2): 249-262.doi: 10.13305/j.cnki.jts.2022.02.004

• Research Paper • Previous Articles     Next Articles

Determination of Multi-pesticide Residues in Tea Based on the Combination of CS/GO/DM SPE Column and UPLC-MS/MS

HU Gaohua1,2,3,4, CAO Jianrong1,3,4, YANG-LEI Wenxuan1,2,3,4, WANG Chen1,3,4, ZHOU Sujuan1,3,4, LIU Xin1,3,4, LU Chengyin1,3,4, CHEN Hongping1,3,4, MA Guicen1,3,4,*   

  1. 1. Tea Research Institute, Chinese Academy of Agriculture Sciences, Hangzhou 310008, China;
    2. Graduate School, Chinese Academy of Agriculture Sciences, Beijing 100081, China;
    3. Key Laboratory of Tea Quality and Safety Control, Ministry of Agriculture and Rural Affairs, Hangzhou 310008, China;
    4. Laboratory of Quality and Safety Risk Assessment for Tea Products (Hangzhou), Ministry of Agriculture and Rural Affairs, Hangzhou 310008
  • Received:2021-12-06 Revised:2022-01-09 Online:2022-04-15 Published:2022-04-15

Abstract: In this study, the synthesized chitosan/graphene oxide/diatomite (CS/GO/DM) solid phase extraction (SPE) column cartridge was used for the purification of tea matrix. A method for determination of 46 kinds of pesticide residues in tea by using ultra-high performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) was established. The tea samples were extracted by acetonitrile, purified by CS/GO/DM SPE column, washed by acetonitrile. Then, the target compounds of 46 pesticide residues were analyzed by UPLC-MS/MS. The results show that CS/GO/DM SPE column could efficiently remove tea interference substances, which could greatly reduce the matrix effect of pesticides in green tea, black tea and oolong tea by 4.7%-66.5%, 3.2%-35.5% and 4.4%-42.8%, respectively. The recoveries of 46 kinds of pesticides ranged from 61.5% to 118% at three spiked levels, with good linearity. The correlation coefficients (R2) were all higher than 0.98. The developed method has the great advantages of operation simplicity, time saving, and high cleanup ability with only one kind towards different types of tea, no need for using matrix standard solution and a low amount of adsorbent.

Key words: CS/GO/DM, matrix purification, pesticide residues, tea, UPLC-MS/MS

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