甘肃陇南和浙江金华绿茶的理化成分及体外抗氧化差异性研究

Abstract

Abstract: To investigate the effects of geographical origin and cultivar on green tea quality, this study analyzed the liquor color, physicochemical components, and in vitro antioxidant activities of eight green teas from Longnan, Gansu and Jinhua, Zhejiang. Correlation and cluster analyses were employed for a comprehensive evaluation. The results show that compared to Longnan green teas, Jinhua green teas exhibited brighter green liquor color and a fresher, sweeter aftertaste. In contrast, Longnan green teas contained higher levels of tea polyphenols, flavonoids, caffeine, vitamin C (VC), catechins, and mineral elements, as well as superior in vitro antioxidant activities, including DPPH radical scavenging ability, ABTS radical scavenging ability, hydroxyl radical (·OH) scavenging ability, and total antioxidant ability (T-AOC). Among the tested cultivars, Longnan Camellia sinensis cv. ‘Yunfeng 5' and Jinhua Camellia sinensis cv. ‘Jiaming 1' had the best overall quality and antioxidant performance. The correlation results show that the color, physical and chemical components and antioxidant capacity of tea decoction were closely correlated. Among them, tea polyphenols, VC and epigallocatechin gallate (EGCG) were positively correlated with the antioxidant ability of the four kinds of tea decoction (P<0.01). Cluster analysis divided the samples into two distinct groups corresponding to their geographical origins, indicating that origin had a greater influence on tea quality than cultivar. This study provided a theoretical foundation for evaluating the effects of geographical origin and cultivar on the antioxidant properties of green tea.

Key words: green tea, tea soup color, physicochemical components, antioxidant, correlation analysis

CLC Number:

S571.1
TS272

WANG Yonghui, WANG Duofeng, LI Xuemin, SHI Tianbin, WU Lidong, LIU Zaiguo, ZHANG Guangzhong, ZHAO Fengyun. Study on the Physicochemical Components and in Vitro Antioxidant Differences of Green Tea from Longnan, Gansu and Jinhua, Zhejiang[J]. Journal of Tea Science, 2025, 45(5): 852-864.

References

[1] Wang J Q, Fu Y Q, Chen J X, et al.Effects of baking treatment on the sensory quality and physicochemical properties of green tea with different processing methods[J]. Food Chemistry, 2022, 380: 132-217. doi: 10.1016/j.foodchem.2022.132217.
[2] Zhao C N, Tang G Y, Cao S Y, et al.Phenolic profiles and antioxidant activities of 30 tea infusions from green, black, oolong, white, yellow and dark teas[J]. Antioxidants, 2019, 8(7): 215. doi: 10.3390/antiox8070215.
[3] Xu J Y, Wang M, Zhao J P, et al.Yellow tea (Camellia sinensis L.), a promising Chinese tea: processing, chemical constituents and health benefits[J]. Food Research International, 2018, 107: 567-577. doi: 10.1016/j.foodres.2018.01.063.
[4] Mi S S, Han S J, Wang M X, et al.Comprehensive analysis of the effect of etiolated tea cultivars and harvest seasons on volatile compounds and in vitro antioxidant capacity in steamed green teas[J]. Food Chemistry: X, 2024, 22: 101-279. doi: 10.1016/j.foodres.2018.01.063.
[5] Xiao K Y, Shi Y T, Liu S S, et al.Compositions and antioxidant activity of tea polysaccharides extracted from different tea (Camellia sinensis L.) varieties[J]. Foods, 2023, 12(19): 3584. doi: 10.3390/foods12193584.
[6] Ramadan G, El-Beih N M, Talaat R M, et al. Anti-inflammatory activity of green versus black tea aqueous extract in a rat model of human rheumatoid arthritis[J]. International Journal of Rheumatic Diseases, 2017, 20(2): 203-213.
[7] Amorim D L M N, Vaz R S, Cesário G, et al. Effect of green tea extract on bone mass and body composition in individuals with diabetes[J]. Journal of Functional Foods, 2018, 40: 589-594. doi: 10.1016/j.jff.2017.11.039.
[8] Baláži A, Sirotkin A V, Földešiová M, et al.Green tea can supress rabbit ovarian functions in vitro and in vivo[J]. Theriogenology, 2019, 127: 72-79. doi: 10.1016/j.theriogenology.2019.01.010.
[9] Yang B, Jiang J, Zhang H, et al.Tea quality estimation based on multi-source information from leaf and soil using machine learning algorithm[J]. Food Chemistry: X, 2023, 20: 100975. doi: 10.1016/j.fochx.2023.100975.
[10] 杜茜雅, 刘馨秋, 卢勇. 长江流域茶叶产地历史变迁及其影响因素[J]. 茶叶科学, 2024, 44(4): 694-706.
Du X Y, Liu X Q, Lu Y.Historical changes and influencing factors of tea producing areas in the Yangtze River Basin[J]. Journal of Tea Science, 2024, 44(4): 694-706.
[11] 王君. ICP-MS法测定陇南绿茶中7种重金属元素含量及健康风险评估[J]. 西北师范大学学报(自然科学版), 2023, 59(4): 89-93, 128.
Wang J.Determination of seven heavy metals in Longnan green tea by ICP-MS and health risk assessment[J]. Journal of Northwest Normal University (Natural Science), 2023, 59(4): 89-93, 128.
[12] 李永娟, 梁慧光, 付国瑞, 等. 甘肃陇南六种茶叶生化成分及微量元素分析[J]. 包装与食品机械, 2019, 37(5): 68-72.
Li Y J, Liang H G, Fu G R, et al.Comparative analysis of biochemical components and trace metal elements of different varieties of tea in Longnan, Gansu Province[J]. Packaging and Food Machinery, 2019, 37(5): 68-72.
[13] 刘春晖, 崔超杰, 孔洁玙, 等. 6种中非不同紫芽茶品质特性及其抗氧化活性研究[J]. 食品安全质量检测学报, 2023, 14(1): 279-286.
Liu C H, Cui C J, Kong J Y, et al.Study on quality characteristics and antioxidant activities of 6 kinds of China-Africa different purple-bud tea[J]. Journal of Food Safety & Quality, 2023, 14(1): 279-286.
[14] Jin S, Li M J, Liu Z Q, et al.Study on the correlation between color and taste of beauty tea infusion and the pivotal contributing compounds based on UV-visible spectroscopy, taste equivalent quantification and metabolite analysis[J]. Food Chemistry: X, 2024, 21: 101192. doi: 10.1016/j.fochx.2024.101192.
[15] Shang A, Luo M, Gan R Y, et al.Effects of microwave-assisted extraction conditions on antioxidant capacity of sweet tea (Lithocarpus polystachyus Rehd)[J]. Antioxidants, 2020, 9(8): 678. doi: 10.3390/antiox9080678.
[16] Xu Q L, Wang L, Li W X, et al.Scented tartary buckwheat tea: aroma components and antioxidant activity[J]. Molecules, 2019, 24(23): 4368. doi: 10.3390/antiox9080678.
[17] Jakubczyk K, Kochman J, Kwiatkowska A, et al.Antioxidant properties and nutritional composition of matcha green tea[J]. Foods, 2020, 9(4): 483. doi: 10.3390/foods9040483.
[18] Xie H, Li X C, Ren Z X, et al.Antioxidant and cytoprotective effects of Tibetan tea and its phenolic components[J]. Molecules, 2018, 23(2): 179. doi: 10.3390/molecules23020179.
[19] 桂安辉, 叶飞, 王胜鹏, 等. 扁形绿茶矿质元素含量与品质成分相关性分析[J]. 食品工业科技, 2022, 43(16): 315-321.
Gui A H, Ye F, Wang S P, et al.Correlation analysis between mineral elements content and quality ingredients of flat green tea[J]. Science and Technology of Food Industry, 2022, 43(16): 315-321.
[20] 史娟, 李江. 微波辅助消解火焰原子吸收光谱法测定茶叶中的微量元素[J]. 江苏农业科学, 2015, 43(11): 380-382.
Shi J, Li J.Determination of trace elements in tea by microwave assisted digestion flame atomic absorption spectrometry[J]. Jiangsu Agricultural Sciences, 2015, 43(11): 380-382.
[21] 陈雅玲, 范新会. 茶园土壤硒含量与茶叶硒含量的相关性分析[J]. 安徽农学通报, 2024, 30(19): 65-69.
Chen Y L, Fan X H.Correlation analysis of selenium content in soil and tea in tea garden[J]. Anhui Agricultural Science Bulletin, 2024, 30(19): 65-69.
[22] 龙飘飘, 苏胜晓, 张梁, 茶叶颜色成分研究进展[J]. 茶叶科学, 2023, 43(5): 593-606.
Long P P, Su S X, Zhang L.Research progress on colored substances in tea[J]. Journal of Tea Science, 2023, 43(5): 593-606.
[23] Li M J, Zhu Y Y, Yuan Y W, et al.Exploring the relationship between color and taste: unveiling critical color and taste compounds of Qingxiang Tieguanyin tea infusion[J]. LWT, 2024, 205: 116456. doi: 10.1016/j.lwt.2024.116456.
[24] 尹军峰, 许勇泉, 陈根生, 等. 影响龙井茶汤品质的主要水质因素分析[J]. 中国茶叶, 2018, 40(6): 20-23.
Yin J F, Xu Y Q, Chen G S, et al.Analysis of major water quality factors affecting the quality of Longjing tea infusion[J]. China Tea, 2018, 40(6): 20-23.
[25] Long P P, Su S X, Han Z S, et al.The effects of tea plant age on the color, taste, and chemical characteristics of Yunnan Congou black tea by multi-spectral omics insight[J]. Food Chemistry: X, 2024, 21: 101-190. doi: 10.1016/j.fochx.2024.101190.
[26] 金亮, 李小白, 丁华, 等. 不同种类茶叶抗氧化活性及茶汤颜色参数比较[J]. 中国食品学报, 2016, 16(2): 242-250.
Jin L, Li X B, Ding H, et al.Comparison on antioxidant activity and color parameters for different types of tea[J]. Journal of Chinese Institute of Food Science and Technology, 2016, 16(2): 242-250.
[27] Wen B, Ren S, Zhang Y Y, et al.Effects of geographic locations and topographical factors on secondary metabolites distribution in green tea at a regional scale[J]. Food Control, 2020, 110: 106979. doi: 10.1016/j.foodcont.2019.106979.
[28] Wang L Y, Wei K, Jiang Y W, et al.Seasonal climate effects on flavanols and purine alkaloids of tea (Camellia sinensis L.)[J]. European Food Research and Technology, 2011, 233(6): 1049-1055.
[29] 肖健, 刘慧, 郑福维, 等. 不同海拔保靖黄金茶品质形成的气象因子初探[J]. 农学学报, 2021, 11(3): 68-73.
Xiao J, Liu H, Zheng F W, et al.Meteorological factors of quality formation of Baojing gold tea at different altitudes: a preliminary study[J]. Journal of Agriculture, 2021, 11(3): 68-73.
[30] 王玉林, 韦美玉, 宋丽莎, 等. 都匀毛尖茶维生素C含量的测定[J]. 山东化工, 2014, 43(12): 82-84.
Wang Y L, Wei M Y, Song L S, et al.Determination of vitamin C in Duyun maojian tea[J]. Shandong Chemical Industry, 2014, 43(12): 82-84.
[31] Lee J E, Lee B J, Chung J O, et al.Geographical and climatic dependencies of green tea (Camellia sinensis) metabolites: a ¹H NMR-Based metabolomics study[J]. Journal of Agricultural and Food Chemistry, 2010, 58(19): 10582-10589.
[32] Choudhury S S, Mukherjee R, Ghosh R, et al.The positive effect of UV radiation on biochemical and microbiological characteristics of different altitude grown Darjeeling tea clones[J]. International Journal of Food Science and Nutrition, 2018, 3(2): 28-31.
[33] Zhao H Y, Yang Q L.The suitability of rare earth elements for geographical traceability of tea leaves[J]. Journal of the Science of Food and Agriculture, 2019, 99(14): 6509-6514.
[34] 罗钦, 陈谢勇, 徐梦婷, 等. 不同海拔区间白茶化学元素特征分析及产地判别[J]. 核农学报, 2024, 38(3): 481-493.
Luo Q, Chen X Y, Xu M T, et al.Characteristic analysis of chemical elements and identification of production regions of white tea from different altitudes intervals[J]. Journal of Nuclear Agricultural Sciences, 2024, 38(3): 481-493.
[35] Deng X F, Liu Z, Zhan Y, et al.Predictive geographical authentication of green tea with protected designation of origin using a random forest model[J]. Food Control, 2020, 107: 106807. doi: 10.1016/j.foodcont.2019.106807.
[36] 姚清华, 颜孙安, 张炳铃, 等. 茶园土壤类型对铁观音茶叶稀土元素分布和组成的影响[J]. 热带亚热带植物学报, 2018, 26(6): 644-650.
Yao Q H, Yan S A, Zhang B L, et al.Effects of soil types in tea garden on distribution and composition of rare earth elements in Tieguanyin[J]. Journal of Tropical and Subtropical Botany, 2018, 26(6): 644-650.
[37] 李梁, 李春霖, 王贞红, 等. 西藏茶叶稳定同位素特征与区域差异及环境因子关系分析[J]. 核农学报, 2022, 36(9): 1797-1804.
Li L, Li C L, Wang Z H, et al.Characteristics of stable isotopes in tibet tea differentiating with other regions and relationship with environment variables[J]. Journal of Nuclear Agricultural Sciences, 2022, 36(9): 1797-1804.
[38] 方洪生, 周迎春, 苏有健. 海拔高度对茶园环境及茶叶品质的影响[J]. 安徽农业科学, 2014, 42(20): 6573-6575.
Fang H S, Zhou Y C, Su Y J.Effects of different elevations of tea gardens on environment and tea quality[J]. Journal of Anhui Agricultural Sciences, 2014, 42(20): 6573-6575.
[39] 黄媛, 于娟, 任玲, 等.不同品种白茶品质及抗氧化活性研究[J]. 食品科技, 2023, 48(7): 64-70.
Hang Y, Yu J, Ren L, et al.Study on the quality and antioxidant activity of different varieties of white tea[J]. Food Science and Technology, 2023, 48(7): 64-70.
[40] Zhang C, Sune L C, Yang C, et al.Antioxidant capacity and major polyphenol composition of teas as affected by geographical location, plantation elevation and leaf grade[J]. Food Chemistry, 2018, 244: 109-119. doi: 10.1016/j.foodchem.2017.09.126.
[41] 师梦楠, 彭云, 张杰, 等. 云南大叶种茶树花生化成分及体外抗氧化活性研究[J]. 食品工业科技, 2022, 43(16): 298-306.
Shi M N, Peng Y, Zhang J, et al.Biochemical components and in vitro antioxidant activities of large leaf species tea flowers in Yunnan[J]. Science and Technology of Food Industry, 2022, 43(16): 298-306.
[42] 张春花, 颜学行, 崔廷宏, 等. 4种云南大叶种白茶主要化学成分及抗氧化活性差异分析[J]. 食品工业科技, 2025, 46(9): 234-242.
Zhang C H, Yan X X, Cui Y H, et al.Comparative analysis on chemical components and antioxidant activity for four different varieties of Yunnan large-leaf white tea[J]. Science and Technology of Food Industry, 2025, 46(9): 234-242.
[43] 霍华珍, 蔡爱华, 郭春雨, 等. 不同品种毛茶水浸出物成分及抗氧化、降血糖活性研究[J]. 广西植物, 2023, 43(6): 1145-1154.
Huo H Z, Cai A H, Guo C Y, et al.Components, antioxidant and hypoglycemic activities of water extracts from different varieties of raw tea[J]. Guihaia, 2023, 43(6): 1145-1154.
[44] Gan R Y, Li H B, Sui Z Q, et al.Absorption, metabolism, anti-cancer effect and molecular targets of epigallocatechin gallate (EGCG): an updated review[J]. Critical Reviews in Food Science and Nutrition, 2018, 58(6): 924-941.
[45] Xu R, Zhu M R, Cao J W, et al.Tea polyphenols protect the mammary gland of dairy cows by enhancing antioxidant capacity and regulating the TGF-beta1/p38/JNK pathway[J]. Metabolites, 2022, 12(11): 1009. doi: 10.3390/metabo12111009.
[46] Klepacka J, Tońska E, Rafałowski R, et al.Tea as a source of biologically active compounds in the human diet[J]. Molecules, 2021, 26(5): 1487. doi: 10.3390/molecules26051487.
[47] Xu J, Zhang Y Y, Zhang M K, et al.Effects of foliar selenium application on Se accumulation, elements uptake, nutrition quality, sensory quality and antioxidant response in summer-autumn tea[J]. Food Research International, 2024, 175: 113618. doi: 10.1016/j.foodres.2023.113618.

Study on the Physicochemical Components and in Vitro Antioxidant Differences of Green Tea from Longnan, Gansu and Jinhua, Zhejiang

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