茶树EST-SSR的信息分析与标记建立

doi:10.13305/j.cnki.jts.2006.01.003

Abstract

Abstract: ：Totally 281 SSRs distributed in 246 ESTs were mined out, accounting for 17.68% of 1589 ESTs updated in tea. The average length of tea plant EST-SSRs searched out is 33.06βbp and the average distance of distribution is 1/2.16βkb. The dinucleotide repeat is the dominant type with repeat motif AG/CT being the most common. 19 primer pairs for EST-SSRs were designed. After testing on the annealing temperature and the concentration of primers, dNTP and MgCl₂, a suitable PCR system was established. Under the condition of reaction system developed, the primers designed were screened against genomic DNA of Longjing 43 from which most EST-SSRs were derived, and 16 primer pairs showed the amplification,accounting for 82.4% of total primers. Then the primers showing amplification were subjected to PCR for DNAs from 10 tea plant cultivars and 10 primer sets showed polymorphisms, accounting for 62.5% of primers available. Results prove that it is an effective and feasible approach to develop SSR markers based on ESTs in tea.

Key words: tea plant, ESTs, SSR information, marker development

CLC Number:

S571.1
Q523

JIN Ji-qiang, CUI Hai-rui, CHEN Wen-yue, LU Mei-zhen, YAO Yan-ling, XIN Ya, GONG Xiao-chun. Data Mining for SSRs in ESTs and Development of EST-SSR Marker in Tea Plant (Camellia sinensis)[J]. Journal of Tea Science, 2006, 26(1): 17-23.

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Data Mining for SSRs in ESTs and Development of EST-SSR Marker in Tea Plant (Camellia sinensis)

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