茶树基因芯片的研制和初步应用

doi:10.13305/j.cnki.jts.2006.03.002

Abstract

Abstract: Totally, 1β680 genes obtained in our EST project were selected from the cDNA library of clone Longjing 43 to develop the first cDNA microarray of tea plant. Each gene in the microarray was duplicated. The cDNA microarray contains 6β912 dots, including 6β720 EST, 160 positive controls and 32 negative controls. One microarray was spotted two regions with 4×4 matrixes and it could be hybridized twice. The density of the microarray was 1037 dots per cm². Three tea clones with different contents of tea polyphenol were selected to conduct two sets of hybridization experiments with the microarray and the differently expressed ESTs were found. Then, two genes that were closely related to tea aroma were selected for real-time PCR analysis to validate the microarray data and validated the reliability of the cDNA microarray. The newly developed cDNA microarray could be applied in various tea research fields to make the high-throughput detection in the gene expression profiling.

Key words: tea plant (Camellia sinensis), cDNA microarray, gene expression profiling, real-time PCR

CLC Number:

S571.1
Q523

ZHAO Li-ping, GAO Qi-kang, CHEN Liang, WANG Xin-chao, YAO Ming-zhe. Development and Preliminary Application of cDNA Microarray of Tea Plant (Camellia sinensis)[J]. Journal of Tea Science, 2006, 26(3): 166-170.

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Development and Preliminary Application of cDNA Microarray of Tea Plant (Camellia sinensis)

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