茯砖茶“金花”菌的分离、鉴定及转化葛根产物研究

doi:10.13305/j.cnki.jts.2016.03.006

Abstract

Abstract: In order to research whether the ′Jinhua′ fungus grow on and biotransformate kudzu root, get higher active fermentation products, one of advantages of strain was isolated from Fuzhuan Tea, named it as LS1. Pueraria lobata, its in the wild as the substrates in solid-state fermentation. DPPH radical scavenging capacities of fermentation products were investigated, making a determination of the contents of total flavone and fermentation products of puerarin were detected by high performance liquid chromatography. The result show that LS1 was identified preliminarily as Eurotium cristatum with GenBank accession number of KR812327 by morphological characteristics and the analysis of ITS-5.8S rDNA region. The contents of total flavone and puerarin were improved 15.97%、12.33%、20.69% and 20.48%、7%、20.29%, respectively.

Key words: ‘Jinhua’ fungus, kudzu root, fermentation, identification, antioxidant activity, puerarin

CLC Number:

HU Xiexin, YI Youjin, BO Lianyang, LI Gaoyang, ZHOU Hongli, ZHOU Jinwei, XIA Bo. Research on Separation, Identification and Transformation Products Transformed Kudzu Root of ′Jinhua′ Fungus[J]. Journal of Tea Science, 2016, 36(3): 268-276.

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Research on Separation, Identification and Transformation Products Transformed Kudzu Root of ′Jinhua′ Fungus

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