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Journal of Tea Science ›› 2012, Vol. 32 ›› Issue (6): 523-529.doi: 10.13305/j.cnki.jts.2012.06.014

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Molecular Cloning and Quantitative Analysis of GDH, GS and GOGAT Genes from Leave of Tea Plant

LIN Zheng-he, ZHONG Qiu-sheng, CHEN Chang-song*   

  1. Tea Research Institute, Fujian Academy of Agricultural Sciences, Fuan 355000, China
  • Received:2012-01-06 Revised:2012-04-01 Online:2012-12-15 Published:2019-09-05

Abstract: This research has separated gene conservative regions of glutamate dehydrogenase (GDH), Glutamine synthetase (GS) and Glutamine oxoglutarate aminotransferase (GOGAT), which were key enzymes involving in the metabolism of amino acid in tea plant, from four tea germplasms. Sequences of GDH, GS and GOGAT conservative regions have been submitted to GeneBank and their accession number were JN602371, JN602372 and JN602373, respectively. Real-time quantitative PCR analysis revealed that GDH gene has higher transcription in 0314C (germplasm with relatively high amino acid content) than the other three germplasms, while lower transcription in 0212-15 (germplasm with relatively low amino acid content) comparing to the others. The transcription of GS and GOGAT gene were in apparently low level in 0314C and high level in 0212-15 and 0318D, respectively. Regression analysis indicated that the expression of GS gene was negatively related to the contents of theanine, lysine and lactamine, whereas the expression of GDH gene was positively related to the content of theanine.

Key words: tea germplasm, gene, clone, fluorescence quantitative PCR

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