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Journal of Tea Science ›› 2018, Vol. 38 ›› Issue (2): 146-154.doi: 10.13305/j.cnki.jts.2018.02.005

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Cloning and Expression Analysis of Small GTPase (CsRAC5) under Cold Stress in Tea Plant (Camellia sinensis)

YE Xiaoli, PAN Junting, ZHU Jiaojiao, SHU Zaifa, CUI Chuanlei, XING Anqi, NONG Shouhua, ZHU Xujun, FANG Wanping, WANG Yuhua*   

  1. College of Horticulture, Nangjing Agricultural University, Nanjing 210095, China
  • Received:2017-10-09 Revised:2017-11-25 Online:2018-04-15 Published:2019-08-28

Abstract: Small GTPase binding proteins are a kind of important signal transduction proteins, which are involved in various life activities of plants. However, few relative studies were reported in tea plants (Camellia sinensis). Here, a small GTPase binding protein named CsRAC5 was cloned by using a cDNA template from tea cultivar ‘Longjingchangye’. The results showed that the length of its open reading frame (ORF) is 597βbp, encoding 198 amino acids. It has a conserved Rho domain which belongs to ROP family. Multiple alignment of CsRAC5 with homologue genes in other plant species showed that their identity could reach 95.96%. CsRAC5 is a hydrophilic protein with the theoretical relative weight of 21.79βkDa. The subcellular assay showed that CsRAC5 was localized in the nuclear and membrane. In addition, the results of RT-PCR analysis showed that the highest expression level of CsRAC5 was in leaves but the lowest in pollen. The expression level of CsRAC5 was decreased under cold stress.

Key words: Camellia sinensis, CsRAC5, bioinformatics analysis, cold stress, expression analysis

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