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Journal of Tea Science ›› 2020, Vol. 40 ›› Issue (3): 341-351.doi: 10.13305/j.cnki.jts.2020.03.005

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Genetic and Phylogenetic Analysis for Resources of Camellia Sinensis from Kaihua County in Zhejiang Province

YU Shuping1, XU Liyi2, WU Rongmei1, WANG Liyuan2, WU Liyun2, WEI Kang2, CHENG Hao2,*, WANG Yongqi1   

  1. 1. Bureau of Agriculture and Rural Affairs of Kaihua, Kaihua 324300, China;
    2. National Centre for Tea Improvement, Tea Research Institute, Chinese Academy of Agricultural Sciences, Hangzhou 310008, China
  • Received:2019-09-18 Revised:2019-10-20 Online:2020-06-15 Published:2020-06-09

Abstract: In this study, various tea resources from Kaihua County were collected to evaluate genetic diversity and phylogenetic relationships among individuals by SSR markers. Meanwhile, suitable core marker combinations were screened to construct fingerprint map. The results show that: (1) 14 SSR markers were polymorphic in the samples. The number of alleles per SSR locus was from 3 to 6 with the mean value of 4.14, and the average number of effective alleles was 3.08. (2) each germplasm resource could be identified by using 14 SSR markers. And based on the analysis of complex loci, the value of PE-1 and PE-3 were over 0.99 and PE-2 over 0.95, respectively, when 10 core SSR loci, as a simplified combination, were successfully screened to distinguish 36 germplasm resources. (3) 36 samples were divided into three groups based on UPGMA phylogenetic tree, and it was preliminarily speculated that five combinations might have parent-child relationship in the sample group through phylogenetic analysis. Present study indicated that tea germplasms in Kaihua County displayed highly diverse genetic backgrounds and might provide useful plant resources for breeding of new cultivars.

Key words: SSR marker, genetic background, phylogenetic analysis, Kaihua County, Camellia sinensis

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