广东苦茶资源嘌呤生物碱含量分析与评价

doi:10.13305/j.cnki.jts.2021.01.008

Abstract

Abstract: The purine alkaloids of 13 bitter tea germplasms from Guangdong Province along with 28 representative cultivars (lines) from different tea producing areas in China were selected for analysis in this paper. The results indicate that genetic diversity index (H′) of purine alkaloids ranged from 0.78 to 1.94, while the coefficient of variation (CV) ranged from 34.89% to 191.19%. The samples could be divided into 6 groups based on cluster analysis: group Ⅰ, Ⅱ and Ⅲ were predominantly consisted of conventional cultivars with high caffeine content; group Ⅴ contained caffeine-free cultivars from Nankun Mountain, while all samples in group Ⅳ and Ⅵ were bitter tea with high theacrine content. A total of 10 purine alkaloid specific bitter tea resources were identified. Moreover, theacrine showed a gradually decrease trend with the increase of leaf positions but maintained a high level in mature leaves of bitter tea. Taken together, our research provided a basis for genetic improvement of functional tea cultivars with specific purine alkaloids in the future.

Key words: bitter tea, purine alkaloids, germplasms, genetic diversity, cluster analysis

CLC Number:

S571.1
S324

LI Hongjian, QIN Dandan, JIANG Xiaohui, FANG Kaixing, WANG Qiushuang, PAN Chendong, LI Bo, WANG Qing, WU Hualing. Analysis and Evaluation of Purine Alkaloid Contents in Bitter Tea Germplasm Resources from Guangdong[J]. Journal of Tea Science, 2021, 41(1): 71-79.

References

[1] 王新超, 姚明哲, 马春雷, 等. 我国苦茶资源主要生化成分的鉴定评价[J]. 中国农学通报, 2008, 24(6): 65-69.
Wang X C, Yao M Z, Ma C L, et al.Analysis and evaluation of biochemical components in bitter tea plant germplasms[J]. Chinese Agricultural Science Bulletin, 2008, 24(6): 65-69.
[2] 漠丽萍. 勐海县苦茶资源现状及开发利用探析[J]. 现代农业科技, 2017, 699(13): 27-28.
Mo L P.Discussion on resource status and development and utilization of Camellia assamica var. kucha in Menghai County[J]. Modern Agricultural Sciences and Technology, 2017, 699(13): 27-28.
[3] 黎娜, 黄怀生, 钟兴刚, 等. 湖南地方特色茶树资源江华苦茶研究进展[J]. 茶叶通讯, 2018, 45(3): 6-10.
Li N, Huang H S, Zhong X G, et al.Research advance on Hunan local tea tree resources Jianghua Bitter Tea [J]. Journal of Tea Communication, 2018, 45(3): 6-10.
[4] 沈程文, 黄意欢, 黄建安, 等. 湖南典型茶树地理种群遗传多样性[J]. 农业生物技术学报, 2007, 15(5): 855-860.
Shen C W, Huang Y H, Huang J A, et al.RAPD analysis for genetic diversity of typical tea populations in Hunan Province[J]. Journal of Agricultural Biotechnology, 2007, 15(5): 855-860.
[5] 李丹, 罗军武. 江华苦茶种质资源的亲缘关系及在茶树进化中的地位研究进展[J]. 湖南农业科学, 2011(1): 18-20.
Li D, Luo J W.Relationship of Thea assamica cv. Jianghua germplasm resources and its status in the process of tea evolution[J]. Hunan Agricultural Science, 2011(1): 18-20.
[6] 张宏达. 茶叶植物资源的订正[J]. 中山大学学报(自然科学版), 1984, 23(1): 3-14.
Zhang H D.A revision on the tea resource plants[J]. Acta Scientiarum Naturalium Universitatis Sunyatseni, 1984, 23(1): 3-14.
[7] 石祥刚, 郑新强, 宋晓虹. 关于苦茶的新组合[J]. 中山大学学报(自然科学版), 2008, 47(6): 129-130.
Shi X G, Zheng X Q, Song X H, et al.A new combination of Kucha[J]. Acta Scientiarum Naturalium Universitatis Sunyatseni, 2008, 47(6): 129-130.
[8] van Dieren S, Uiterwaal C S P M, van der Schouw Y T, et al. Coffee and tea consumption and risk of type 2 diabetes[J]. Diabetologia, 2009, 52(12): 2561-2569.
[9] Heckman M A, Weil J, Mejia E G D. Caffeine (1,3,7-trimethylxanthine) in foods: a comprehensive review on consumption, functionality, safety, and regulatory matters[J]. Journal of Food Science, 2010, 75(3): 77-87.
[10] Li Y F, Chen M, Wang C, et al.Theacrine, a purine alkaloid derived from Camellia assamica var. kucha, ameliorates impairments in learning and memory caused by restraint-induced central fatigue[J]. Journal of Functional Foods, 2015, 16: 472-483.
[11] Qiao H Y, Ye X S,Bai X Y, et al.Theacrine: a purine alkaloid from Camellia assamica var. kucha with a hypnotic property via the adenosine system[J]. Neuroscience Letters, 2017, 659: 48-53.
[12] 龚频, 石富兄, 陈福欣, 等. 云南苦茶提取物抗氧化应激保护镉致糖尿病肾病小鼠的作用[J]. 现代食品科技, 2019, 35(8): 6-12.
Gong P, Shi F X, Chen F X, et al.Anti-oxidative stress of Yunnan bitter tea extract protects mice in high fat-cadmium induced diabetic nephropathy[J]. Modern Food Science and Technology, 2019, 35(8): 6-12.
[13] 宛晓春. 茶叶生物化学[M]. 3版. 北京: 中国农业出版社, 2013.
Wan X C.Tea Biochemistry [M]. 3rd ed. Beijing: China Agriculture Press, 2013.
[14] 杨春, 罗军武, 陈涛林, 等. 高咖啡碱含量的特异茶树种质资源的筛选[J]. 湖南农业科学, 2013(17): 9-12.
Yang C, Luo J W, Chen T L, et al.Screening specific germplasm resources of tea plant with high caffeine content[J]. Hunan Agricultural Sciences, 2013(17): 9-12.
[15] 唐琴, 孙威江, 陈志丹, 等. 尤溪苦茶资源苦涩味物质测定与分析[J]. 食品科学, 2019, 40(18): 242-247.
Tang Q, Sun W J, Chen Z D, et al.Determination and analysis of bitter and astringent substances in Youxi bitter tea resources[J]. Food Science, 2019, 40(18): 242-247.
[16] 叶创兴, 林永成, 苏建业, 等. 苦茶Camellia assamica var. kucha Chang et Wang的嘌呤生物碱[J]. 中山大学学报(自然科学版), 1999, 38(5): 82-86.
Ye C X, Lin Y C, Su J Y, et al.Purine alkaloids in camellia assamica var. kucha Chang et Wang[J]. Acta Scientiarum Naturalium Universitatis Sunyatseni, 1999, 38(5): 82-86.
[17] 叶创兴, Ashihara Hiroshi, 郑新强, 等. 一种野生茶树的新嘌呤碱模式[J]. 中山大学学报(自然科学版), 2003, 42(1): 62-65.
Ye C X, Ashihara H, Zheng X Q, et al.New discovery of pattern of purine alkaloids in wild tea trees[J]. Acta Scientiarum Naturalium Universitatis Sunyatseni, 2003, 42(1): 62-65.
[18] 杨超, 马存强, 周斌星, 等. 苦茶特征性成分研究[J]. 昆明学院报, 2014, 36(3): 75-78.
Yang C, Ma C Q, Zhou B X, et al.Characteristic components research in bitter tea[J]. Journal of Kunming University, 2014, 36(3): 75-78.
[19] 黄华林, 李家贤, 何玉娟, 等. 广东茶树种质资源保存与利用现状[J]. 广东茶业, 2006(5): 14-17.
Huang H L, Li J X, He Y J, et al.The current status of tea plant germplasm conservation and utilization in Guangdong Province[J]. Guangdong Tea Industry, 2006(5): 14-17.
[20] 吴华玲, 陈栋, 李家贤. 广东特异茶树种质资源选育研究进展[J]. 广东农业科学, 2012(20): 15-17, 24.
Wu H L, Chen D, Li J X.The investigation and reflection on “late spring coldness” injury of Guangdong tea area[J]. Guangdong Agricultural Sciences, 2012(20): 15-17, 24.
[21] 陈亮, 杨亚军, 虞富莲, 等. 茶树种质资源描述规范和数据标准[M]. 北京: 中国农业出版社. 2005.
Chen L, Yang Y J, Yu F L, et al.Descriptors and data standard for tea (Camellia Spp.) [M]. Beijing: China Agriculture Press, 2005.
[22] 相吉山, 徐峰, 索良喜, 等. 东北地区谷子地方品种和育成品种表型比较分析[J]. 植物遗传资源学报, 2018, 19(4): 642-656.
Xiang J S, Xu F, Suo L X, et al.Comparison on the phenotype traits between landrace and caltivars of foxtail millet [Setaria italica (L.) P. Beauv] in northeast China[J]. Journal of Plant Genetic resources, 2018, 19(4): 642-656.
[23] 马小卫, 马永利, 武红霞, 等. 基于因子分析和聚类分析的杧果种质矿质元素含量评价[J]. 园艺学报, 2018, 45(7): 1371-1381.
Ma X W, Ma Y L, Wu H X, et al.Assessment of mineral elements contents at the mango germplasm level based on factor analysis and cluster analysis[J]. Acta Horticulturae Sinica, 2018, 45(7): 1371-1381.
[24] 陈常颂, 陈志辉, 钟秋生, 等. 闽育茶树品种生育期与生化成分比较及遗传多样性分析[J]. 茶叶科学, 2014, 34(2): 172-179.
Chen C S, Chen Z H, Zhong Q S, et al.Comparison of growth period and biochemical composition on Fujian tea cultivars and genetic diversity analysis[J]. Journal of Tea Science, 2014, 34(2): 172-179.
[25] 李斌, 陈国本, 陈娟, 等. 茶树天然无咖啡碱珍稀种质资源的研究[J]. 广东茶业, 2000(4): 7-11.
Li B, Chen G B, Chen J, et al.Analysis of a natural caffeine-free tea plant[J]. Guangdong Tea Industry, 2000(4): 7-11.
[26] 金基强, 周晨阳, 马春雷, 等. 我国代表性茶树种质嘌呤生物碱的鉴定[J]. 植物遗传资源学报, 2014, 15(2): 279-285.
Jin J Q, Zhou C Y, Ma C L, et al.Identification on purine alkaloids of representative tea germplasm in China[J]. Journal of Plant Genetic Resources, 2014, 15(2): 279-285.
[27] 滕杰, 曾贞, 黄亚辉. 秃房茶嘌呤生物碱组成特点及生化品质成分的研究[J]. 广西植物, 2018, 38(5): 26-34.
Teng J, Zeng Z, Huang Y H.Composition characteristics of purine alkaloids and biochemical components of Camellia gymnogyna[J]. Guihaia, 2018, 38(5): 26-34.
[28] 王熙. 茶树叶片中主要特征物质的年变化规律研究[D]. 合肥: 安徽农业大学, 2013.
Wang X.A study on annual variation characteristics of the major compounds in tea leaves [D]. Hefei: Anhui Agricultural University, 2013.
[29] Zheng X Q, Ye C X, Kato M, et al.Theacrine (1,3,7,9-tetramethyluric acid) synthesis in leaves of a Chinese Tea, kucha (Camellia assamica var. kucha)[J]. Phytochemistry, 2002, 60(2): 129-134.
[30] Ashihara H, Sano H, Crozier A.Caffeine and related purine alkaloids: biosynthesis, catabolism, function and genetic engineering[J]. Phytochemistry, 2008, 69(4): 841-856.

Analysis and Evaluation of Purine Alkaloid Contents in Bitter Tea Germplasm Resources from Guangdong

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Metrics

Comments

Recommended 0

[1]	CHEN Shichun, JIANG Hongyan, LIAO Shuran, CHEN Tingxu, NIU Jinzhi, WANG Xiaoqing. Genetic Diversity Analysis of Euproctis pseudoconspersa and Its Bunyavirus (EpBYV) in China [J]. Journal of Tea Science, 2024, 44(5): 793-806.
[2]	CHEN Shichun, JIANG Hongyan, LIAO Shuran, CHEN Tingxu, WANG Xiaoqing. Analysis of Genetic Diversity and Genetic Structure in Geographic Populations of Stephanitis chinensis from China Based on Mitochondrial DNA COI Sequence [J]. Journal of Tea Science, 2023, 43(6): 795-805.
[3]	MENG Rongjun, CHEN Liang, XU Yuan, LIN Wei, ZHOU Qiwei, XIE Yilin, LAI Dingqing, LAI Jiaye. Genetic Diversity Analysis of Tea Genetic Resources in Sanjiang, Guangxi [J]. Journal of Tea Science, 2023, 43(2): 147-158.
[4]	LIU Haoran, ZHANG Chenyu, GONG Yang, YE Yuanyuan, CHEN Jiedan, CHEN Liang, LIU Dingding, MA Chunlei. Development and Application of Albinotea Plant mSNP Molecular Markers Based on Genome-wide Resequencing [J]. Journal of Tea Science, 2023, 43(1): 27-39.
[5]	WANG Liubin, HUANG Liyun, TENG Cuiqin, WU Liyun, CHENG Hao, YU Cuiping, WANG Liyuan. Genetic and Phylogenetic Analysis for Germplasm Resources of Camellia sinensis from Wuzhou City [J]. Journal of Tea Science, 2022, 42(5): 601-609.
[6]	CHEN Qiyu, MA Jianqiang, CHEN Jiedan, CHEN Liang. Genetic Diversity of Mature Leaves of Tea Germplasms Based on Image Features [J]. Journal of Tea Science, 2022, 42(5): 649-660.
[7]	LI Changle, GE Yue, YAN Meilin, LI Hui, LIN Qingqing, WANG Pu, ZHAO Hua, WANG Mingle, WANG Yu, GUO Fei, NI Dejiang. Analysis of Genetic Diversity and Population Structure of 32 Tea Landraces in China [J]. Journal of Tea Science, 2021, 41(5): 619-630.
[8]	MAO Juan, JIANG Hongjian, YANG Rubing, LI Chongxing, MA Chengying, CHEN Liang, MA Jianqiang. Genetic Diversity and Population Structure of Wild and Cultivated Camellia taliensis Populations [J]. Journal of Tea Science, 2021, 41(4): 454-462.
[9]	WEI Shasha, PENG Jing, CHEN Zhidan, SUN Weijiang, LIN Lin. Detection of Bitterness-related Substances in Youxi Bitter Tea and Correlation Analysis with Bitterness [J]. Journal of Tea Science, 2021, 41(3): 337-349.
[10]	LIU Zhen, CHENG Yang, YANG Peidi, ZHAO Yang, NING Jing, YANG Yang. Genetic Diversity and Structure of Chengbudong Tea Population Revealed by nSSR and cpDNA Markers [J]. Journal of Tea Science, 2020, 40(2): 250-258.
[11]	DING Zhou, LI Yexin, YUAN Yi, WANG Haiyan, LIU Xueshi, JIANG Changjun. Phenotypic Traits and Genetic Diversity of Elite Tea Population in Anhui（China） [J]. Journal of Tea Science, 2018, 38(2): 155-161.
[12]	MAO Juan, JIANG Hongjian, LI Chongxing, MA Jianqiang, CHEN Liang. Genetic Diversity Analysis of Tea Plant in Baiyingshan Mountain of Yunnan [J]. Journal of Tea Science, 2018, 38(1): 69-77.
[13]	LIN Weidong, CHEN Zhidan, SUN Weijiang, YANG Ruxing. Analysis of Genetic Diversity of Fujian Tea Varieties by SCoT Markers [J]. Journal of Tea Science, 2018, 38(1): 43-57.
[14]	XIA Fagang, ZHONG Xingwang, WU Feng, JI Biaojun. SRAP Marker Analysis of Genetic Diversity and Relationship in Wuyi Rock Tea Germplasm Resources [J]. Journal of Tea Science, 2017, 37(1): 78-85.
[15]	MA Cunqiang, ZHOU Binxing, YANG Chao, WANG Hongzhen, XU Zhengwen. Identification of Spring Sun-dried Tea from Jingmai Area by HPLC Fingerprint [J]. Journal of Tea Science, 2016, 36(4): 379-388.