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茶叶科学 ›› 2007, Vol. 27 ›› Issue (3): 259-264.doi: 10.13305/j.cnki.jts.2007.03.007

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利用cDNA-AFLP技术研究茶树花蕾发育基因差异表达片段

余梅,江昌俊*,叶爱华,王朝霞,朱林,邓威威,高轩,宛晓春   

  1. 安徽农业大学茶叶生物化学和生物技术教育部、农业部重点实验室,安徽 合肥 230036
  • 出版日期:2007-09-25 发布日期:2019-09-11
  • 通讯作者: *江昌俊,jiangcj@ahau.edu.cn
  • 作者简介:余梅(1968— ),女,副教授,博士,从事茶树遗传育种与分子生物学研究。
  • 基金资助:
    安徽省自然科学基金项目(050410102)

Analysis of Differential Gene Expression of Flower bud of Tea Plant (Camellia sinensis) by cDNA-AFLP

YU Mei, JIANG Chang-jun*, YE Ai-hua, WANG Zhao-xia, ZHU Lin, DENG Wei-wei, GAO Xuan, WAN Xiao-chun   

  1. Key Laboratory of Tea Biochemistry & Biotechology of Ministry of Agriculture ﹠ Ministry of Education, Anhui Agricultural University, Hefei, 230036, China
  • Online:2007-09-25 Published:2019-09-11

摘要: 利用cDNA-AFLP技术,对茶树花蕾发育期的基因表达进行了分析。结果表明,茶树花蕾在发育早期和晚期基因的表达中有明显差异,共显示1 110条带,早期发育花蕾和晚期发育花蕾显示的特异条带分别为35条和87条;选取特异表达的晚期发育花蕾重复性较好的15个TDFs(Transcript Derived Fragment)在GenBank进行序列分析,结果显示,9个分别与氧化还原酶、染色体13、14-3-3蛋白、Amyrel基因、钙网蛋白、ATP硫化酶、接触反应/铁离子结合蛋白及花粉特异蛋白的序列有相似性;2个与假拟蛋白相似,4个在GenBank数据库中未找到相似序列。

关键词: 花蕾发育, cDNA-AFLP, 茶树, 基因差异表达

Abstract: For studies of differential gene expression, cDNA-AFLP was analyzed in tea plant at the stage of bud flower development. The result showed that there were evident differential expression in flower between the early stage and the late stage. Of the 1110 fragments inspected, 35 and 87 specially displayed in the early and late stages; 15 fragments were cloned and sequenced. Sequence analysis indicated that among 15 squenced fragments, nine were sequence-similar to proteins in GenBank, i.e. oxidoreductase, chromosome 13, 14-3-3 protein, Amyrel gene, calreticulin interacted protein, ATP sulfurylase, catalytic / iron ion binding, desiccation-related protein and ntp302, two were similar to hypothetical proteins, and no similar sequence were found in GenBank for the other 4 fragment.

Key words: bud flower development, cDNA-AFLP, tea plant(Camellia sinensis(L.)O. Kuntze cv. Wulong), gene differential expression

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