镉胁迫对蒙山茶树生长及叶片生理指标的影响

doi:10.13305/j.cnki.jts.2008.01.011

摘要/Abstract

摘要： 通过盆栽试验,研究不同镉浓度对蒙山茶树生长及叶片生理特性的影响。结果表明：低浓度镉对茶树无明显毒害作用,当镉浓度达到60 mg/kg时,茶树植株受毒害基本死亡;春茶叶绿素含量加镉处理大多高于对照,其增加量先高后低;夏茶叶绿素总量随镉浓度的增加极显著降低,叶绿素含量与添加镉的量呈显著负相关,其相关系数r=–0.997**;春茶、夏茶的丙二醛（Malondialdehyde,MDA）含量随镉处理浓度增加显著增加,其正相关系数分别为r=0.970**,r=0.994**（P<0.01）;春茶游离脯氨酸含量随添加镉浓度的增加显著增加,相关系数r=0.829*（P<0.05）,夏茶游离脯氨酸在镉试验浓度下极显著增加,其相关系数r=0.980**（P<0.01）,且夏茶游离脯氨酸含量明显高于春茶;春茶可溶性糖都高于对照,其增加量先高后低.,相关系数为r= -0.342;夏茶可溶性糖呈下降趋势,其相关系数r= -0.909*（P<0.05）。

关键词: 镉, 茶树, 叶绿素, 丙二醛, 游离脯氨酸, 可溶性糖

Abstract: Effects of different cadmium concentrations on growth of tea plant and physiological index in leaves of Mengshan tea were studied with the tea cultured in pots. Results showed that, low concentration of cadmium has no obvious adverse effect on the tea plant. When the cadmium concentration reached 60 mg/kg,the tea plant harmed to die; The chlorophyⅡ(ChⅠ) content of most spring tea supplemented with Cd was higher than CK and the increasing trend was raised firstly and inhibited later. The chlorophyⅡ content of summer tea supplemented with Cd was significantly decreased with increase of cadmium concentration, and the chlorophyⅡ content showed a negative relationship with the cadmium concentration, and the coefficient of correlation was -0.997**. The malondialdehyde (MDA) content in spring and summer tea was significantly enhanced with the increase of cadmium concentration. The coefficient of correlation were 0.970**,0.994**（P<0.01）respectively. The free proline of spring tea was significantly enhanced wiyh the increase of Cd concentration, the coefficient of correlation was 0.829*（P<0.05）, the free proline in summer tea was significantly enhanced, and the coefficient of correlation was 0.980**（P<0.01）,and the content of free praline in summer tea was higher than that in spring tea. The soluble sugar content in spring tea supplemented with Cd was higher than CK and the increasing trend raised firstly and then inhibited, the coefficient of correlation was -0.342;The soluble sugar content of summer tea supplemented with Cd was significantly decreased and the coefficient of correlation was -0.909*（P<0.05）.

Key words: cadmium, tea plant (Camellia sinensis), Ch Ⅰ, MDA, free proline, soluble sugar

中图分类号:

夏建国, 兰海霞. 镉胁迫对蒙山茶树生长及叶片生理指标的影响[J]. 茶叶科学, 2008, 28(1): 56-61. doi: 10.13305/j.cnki.jts.2008.01.011.

XIA Jian-guo, LAN Hai-xia. Effects of Cadmium Stress on Growth of Tea Plant and Physiological Index in Leaves of Mengshan Tea[J]. Journal of Tea Science, 2008, 28(1): 56-61. doi: 10.13305/j.cnki.jts.2008.01.011.

参考文献

[1] 江用文, 陈宗懋, 鲁成银. 我国茶叶安全质量现状与建议[J]. 中国农业科技导报, 2002, 4(5): 24~27.
[2] 石元值, 吴洵. 当前我国茶叶中铅含量现状及几点建议[J]. 茶叶, 2000, 26(3): 128~129.
[3] 王焕校. 污染生态学基础[M]. 昆明: 云南大学出版社, 1990.
[4] Cieslinski G, Neilser G H, Hogue E J.Effect of soil cadmium application and pH on growth and cadmium accumulation in roots, lesves and fruit of strawberry plants[J]. Plant and Soil, 1996, 180: 67~276.
[5] 杨居荣, 贺建群, 蒋婉如. 镉污染对植物生理生化的影响[J]. 农业环境保护, 1995, 14(5): 193~197.
[6] 程杰, 高压军. 镉毒害对小麦生理生态效应的研究进展[J]. 水土保持研究, 2006, 13(6): 219~221.
[7] 高建兰, 刘玲, 张洪林. 镉胁迫对玉米生理特性的影响[J]. 浙江科技学院学报, 2004, 16(2): 105~108.
[8] 曾路生, 廖敏, 黄昌勇, 等. 镉污染对水稻土微生物量、酶活性及水稻生理指标的影响[J]. 应用生态学报, 2005, 16(11): 2162~216.
[9] Bazaar F A, Rolfe G L, Garlson R W.The effect of cadmium on photosynthesis and transpiration of excised leaves of corn and sunflower[J]. Physiol Plant, l974, 32: 373~377.
[10] Lee K C, Cuningham B A, Paulsen G M, et a1. Effects of cadmium on respiration rate and activities of several enzymes in
[11] soybean seedlings[J]. Physiol Plant, 1976, 36: 4~6.
[12] Lakshamu K C, Virinder K G, Surinder K S.Effect of cadmium on enzyme of nitrogen metabolism in pea seedling[J]. Phytochemistry, 1992, 31(2): 395~400.
[13] 奉天才, 吴玉树, 王焕校, 等. 镉、铅及其相互作用对小白菜根系生理生态效应的研究[J]. 生态学报, 1998, 18(3): 320~325.
[14] 熊庆娥. 植物生理学实验教程[M]. 成都: 四川科学技术出版社, 2003.
[15] 王晶英, 敖红, 张杰, 等. 植物生理生化实验技术与原理[M]. 哈尔滨: 东北林业大学出版社, 2003.
[16] 何翠屏. 两种重金属胁迫对两种草坪草生长与代谢的影响[D]. 武汉: 华中农业大学, 2005.
[17] Nag P, Paul A K, Mukherji S.Heavy metal effects in plant tissues involving chlorophyll, chlorophyllase, hill reaction activity & gel electrophoretic patterns of soluble proteins[J]. Indian journal of experimental biology. 1981, 19(8): 702~706.
[18] 石元值. 茶树中铅元素的吸收累积特性及污染来源研究[D]. 杭州: 浙江大学, 2003.
[19] 刘秀梅, 聂俊华, 王庆仁. Pb对农作物的生理生态效应[J]. 农业环境保护, 2002, 21(3): 201~203.
[20] Pang J, Chan G S Y, Zhang J, et a1. Physiological aspects of vetiver grass for rehabilitation in abandoned metalliferous mine wastes[J]. Chemosphere, 2003, 52: 1559~1570.
[21] Csonka I N.Physiological and genetic responses of bacteria to osmotic stress[J]. Microbia1 Rev, 1989, 53: 121~147.