茶悬浮培养细胞玻璃化超低温保存研究

刘亚军; 高丽萍; 夏涛; 高可君

doi:10.13305/j.cnki.jts.2009.2.006

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茶叶科学 ›› 2009, Vol. 29 ›› Issue (2) : 120-126. DOI: 10.13305/j.cnki.jts.2009.2.006

茶悬浮培养细胞玻璃化超低温保存研究

刘亚军¹, 高丽萍^2,*, 夏涛^1,*, 高可君¹

作者信息 +

Study on Cryopreservation of Suspension Culture Cells by Vitrification in Camellia Sinensis

LIU Ya-jun¹, GAO Li-ping^2,*, XIA Tao^1,*, GAO Ke-jun¹

Author information +

文章历史 +

摘要

为保存具有稳定代谢能力的优良茶细胞系,本文对茶叶悬浮培养细胞玻璃化法超低温保存进行了初步研究。结果表明：预培养以4 d最佳,60% PVS₂冰浴装载以20 min最好,100% PVS₂冰浴脱水处理60 min最优,40℃复温解冻细胞存活率最高,采用这些参数进行完整试验的细胞存活率达到76%,初步建立了茶悬浮培养细胞玻璃化超低温保存的冷冻程序。

Abstract

To preserve eminent cell lines with stable metabolism, the cryopreservation of suspension culture cells by vitrification in Camellia sinensis has been preliminarily investigated in this article. It showed that the preculture for four days was best, loading with 60% PVS₂ in freezing bath for 20 minutes was optimal. Treating with 100% PVS₂ dehydration in freezing bath for 60 minutes was suggested. The cryopreservation of suspension culture cells warmed in 40℃water bath had the highest survival rate. The cells’ survival rate was as high as 76% when these parameters were adopted through the whole trial. The procedure of cryopreservation of suspension culture cells by vitrification in Camellia sinensis was preliminarily established.

导出引用

刘亚军, 高丽萍, 夏涛, 高可君. 茶悬浮培养细胞玻璃化超低温保存研究[J]. 茶叶科学. 2009, 29(2): 120-126 https://doi.org/10.13305/j.cnki.jts.2009.2.006

LIU Ya-jun, GAO Li-ping, XIA Tao, GAO Ke-jun. Study on Cryopreservation of Suspension Culture Cells by Vitrification in Camellia Sinensis[J]. Journal of Tea Science. 2009, 29(2): 120-126 https://doi.org/10.13305/j.cnki.jts.2009.2.006

中图分类号： TS272 Q942

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