基于EST-SSR的福云（半）同胞系茶树品种（系）遗传多样性和亲缘关系分析

余继忠; 黄海涛; 姚明哲; 杨亚军

doi:10.13305/j.cnki.jts.2010.03.006

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茶叶科学 ›› 2010, Vol. 30 ›› Issue (3) : 184-190. DOI: 10.13305/j.cnki.jts.2010.03.006

基于EST-SSR的福云（半）同胞系茶树品种（系）遗传多样性和亲缘关系分析

余继忠^1,2, 黄海涛², 姚明哲¹, 杨亚军^1,*

作者信息 +

Analysis of Genetic Diversity and Relationship of Half-sib Tea Cultivars Related to Fuding Dabai and Yunnan Daye Using EST-SSR Markers

YU Ji-zhong^1,2, HUANG Hai-tao², YAO Ming-zhe¹, YANG Ya-jun^1,*

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文章历史 +

摘要

利用EST-SSR标记对以福鼎大白茶或云南大叶茶品种为亲本选育的40个品种（系）或衍生品种（系）的亲缘关系和遗传背景进行了分析评估。28对引物在40个供试品种间共检测到99个等位位点,每对引物为2~6个,平均3.54个;遗传多态性信息量(PIC)在0.13~0.79之间,平均值为0.59;期望杂合度（He）在0.08~0.84之间,平均值为0.58;观测杂合度(Ho)在0.10~1.00之间,平均值为0.71;Shannon指数平均为1.14。按相似系数为0.55可将参试的40个品种分为四大类。研究结果发现以云南大叶茶为母本的茶树品种比福鼎大白茶为母本的品种遗传多样性更高。

Abstract

40 half-sib tea cultivars related to Fuding Dabai and Yunnan Daye were used to analyze the genetic diversity and relationship based on EST-SSR markers. Totally 99 alleles were amplified using the 28 pairs of EST-SSR primers, the number of alleles per primer ranged from 2 to 6, on average of 3.54. The polymorphism information content (PIC) varied from 0.13 to 0.79, on average of 0.59. The expected heterozygosity (He) varied from 0.08 to 0.84 and the observed heterozygosity (Ho) ranged from 0.08 to 1.00, on average of 0.59 and 0.71, respectively. The Shannon index of the population was 1.14. The 40 half-sib tea cultivars were classified into 4 groups with the similarity coefficient at 0.55. The results showed that the genetic polymorphism of half-sib tea cultivars related to Fuding Dabai was more abundant than those of Yunnan Daye.

导出引用

余继忠, 黄海涛, 姚明哲, 杨亚军. 基于EST-SSR的福云（半）同胞系茶树品种（系）遗传多样性和亲缘关系分析[J]. 茶叶科学. 2010, 30(3): 184-190 https://doi.org/10.13305/j.cnki.jts.2010.03.006

YU Ji-zhong, HUANG Hai-tao, YAO Ming-zhe, YANG Ya-jun. Analysis of Genetic Diversity and Relationship of Half-sib Tea Cultivars Related to Fuding Dabai and Yunnan Daye Using EST-SSR Markers[J]. Journal of Tea Science. 2010, 30(3): 184-190 https://doi.org/10.13305/j.cnki.jts.2010.03.006

中图分类号： S571 Q32

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