川、渝地方茶树品种的遗传多样性和群体结构

姚明哲, 马春雷, 金基强, 马建强, 陈亮

茶叶科学 ›› 2012, Vol. 32 ›› Issue (5) : 419-425.

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茶叶科学 ›› 2012, Vol. 32 ›› Issue (5) : 419-425. DOI: 10.13305/j.cnki.jts.2012.05.011

川、渝地方茶树品种的遗传多样性和群体结构

  • 姚明哲, 马春雷, 金基强, 马建强, 陈亮*
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Genetic Diversity and Population Structure of Local Tea Landraces in Sichuan and Chongqing Revealed by EST-SSR Markers

  • YAO Ming-zhe, MA Chun-lei, JIN Ji-qiang, MA Jian-qiang, CHEN Liang*
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文章历史 +

摘要

利用自主开发的EST-SSR标记分析了四川(川)和重庆(渝)的90份地方茶树品种资源的遗传多样性和群体结构。84对EST-SSR引物在供试样本中共扩增出275个等位位点,平均每对引物可鉴定3.3个等位位点,平均位点杂合度为0.33。90份地方品种的平均基因多样性(H)和多态性信息含量(PIC)分别为0.462、0.407。比较分析表明,重庆地方茶树品种的遗传多样性略高于四川,川、渝南部(Lat.

Abstract

Ninety local tea landraces collected from Sichuan and Chongqing provinces were used to analyze genetic diversity and population structure based on 84 EST-SSR markers. A total of 275 alleles were amplified, with an average of 3.3 alleles and 0.33 of heterozygosity per locus. The mean gene diversity (H) and polymorphic information content (PIC) were estimated to be 0.462 and 0.407, respectively, among 90 tested accessions. The level of genetic diversity among local tea landraces in Chongqing was higher than that in Sichuan. And comparing to tea landraces from north part (Lat.≥N32°), the genetic diversity was significant higher in the southern part (Lat.

关键词

EST-SSR / 茶树 / 地方品种 / 群体结构 / 遗传多样性

Key words

Camellia sinensis / EST-SSR / genetic diversity / landrace / population structure

引用本文

导出引用
姚明哲, 马春雷, 金基强, 马建强, 陈亮. 川、渝地方茶树品种的遗传多样性和群体结构[J]. 茶叶科学. 2012, 32(5): 419-425 https://doi.org/10.13305/j.cnki.jts.2012.05.011
YAO Ming-zhe, MA Chun-lei, JIN Ji-qiang, MA Jian-qiang, CHEN Liang. Genetic Diversity and Population Structure of Local Tea Landraces in Sichuan and Chongqing Revealed by EST-SSR Markers[J]. Journal of Tea Science. 2012, 32(5): 419-425 https://doi.org/10.13305/j.cnki.jts.2012.05.011
中图分类号: S571.1    S330   

参考文献

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基金

国家茶叶产业技术体系(CARS-023)、作物种质资源保护项目(NB2011-2130135)、浙江省自然科学基金(Y3110260)

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