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茶叶科学 ›› 2012, Vol. 32 ›› Issue (5): 419-425.doi: 10.13305/j.cnki.jts.2012.05.011

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川、渝地方茶树品种的遗传多样性和群体结构

姚明哲, 马春雷, 金基强, 马建强, 陈亮*   

  1. 中国农业科学院茶叶研究所国家茶树改良中心,浙江 杭州 310008
  • 收稿日期:2012-03-19 修回日期:2012-04-23 出版日期:2012-10-15 发布日期:2019-09-05
  • 通讯作者: liangchen@mail.tricaas.com
  • 作者简介:姚明哲(1975— ),男,山东荷泽人,农学博士,副研究员,主要从事茶树种质鉴定、发掘和创新利用研究。
  • 基金资助:
    国家茶叶产业技术体系(CARS-023)、作物种质资源保护项目(NB2011-2130135)、浙江省自然科学基金(Y3110260)

Genetic Diversity and Population Structure of Local Tea Landraces in Sichuan and Chongqing Revealed by EST-SSR Markers

YAO Ming-zhe, MA Chun-lei, JIN Ji-qiang, MA Jian-qiang, CHEN Liang*   

  1. National Center for Tea Improvement, Tea Research Institute of the Chinese Academy of Agricultural Sciences, Hangzhou 310008, China
  • Received:2012-03-19 Revised:2012-04-23 Online:2012-10-15 Published:2019-09-05

摘要: 利用自主开发的EST-SSR标记分析了四川(川)和重庆(渝)的90份地方茶树品种资源的遗传多样性和群体结构。84对EST-SSR引物在供试样本中共扩增出275个等位位点,平均每对引物可鉴定3.3个等位位点,平均位点杂合度为0.33。90份地方品种的平均基因多样性(H)和多态性信息含量(PIC)分别为0.462、0.407。比较分析表明,重庆地方茶树品种的遗传多样性略高于四川,川、渝南部(Lat.<N30°)地方茶树品种的多样性明显高于北部(Lat.≥N32°)。基于数学模型和Nei遗传距离的聚类分析均表明,川、渝茶树地方品种在群体结构上有较明显的差异,表明其可能具有不同的演化和传播途径。

关键词: 茶树, 地方品种, 遗传多样性, 群体结构, EST-SSR

Abstract: Ninety local tea landraces collected from Sichuan and Chongqing provinces were used to analyze genetic diversity and population structure based on 84 EST-SSR markers. A total of 275 alleles were amplified, with an average of 3.3 alleles and 0.33 of heterozygosity per locus. The mean gene diversity (H) and polymorphic information content (PIC) were estimated to be 0.462 and 0.407, respectively, among 90 tested accessions. The level of genetic diversity among local tea landraces in Chongqing was higher than that in Sichuan. And comparing to tea landraces from north part (Lat.≥N32°), the genetic diversity was significant higher in the southern part (Lat.<N30°) of Sichuan and Chongqing. Meanwhile, this study revealed that there was a significant difference between Sichuan and Chongqing populations according to clustering analysis based on either statistics model or Nei’ genetic distance. It indicated the origin and spread of local tea landraces might be different in Sichuan and Chongqing.

Key words: Camellia sinensis, landrace, genetic diversity, population structure, EST-SSR

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