调节pH值对HSCCC分离茶黄素的影响

doi:10.13305/j.cnki.jts.2003.s1.017

Abstract

Abstract: The four main kinds of theaflavins could be separated into three parts with the solvent system (Ethyl acetate / Methanol / Hexane / Water, 3/1/1/6) on HSCCC. The different pH zone of elutes could be appeared in the case of the difference of pH between sample solution and mobile phase. Due to the fact of the separation and purification of theaflavins are influenced by the pH of solution, and the process of separation and eluation is closely related to the pH of solution, so the method of modifying pH could be used to highly concentrate the four main kinds of theaflavins from the large scale of samples.

Key words: Key Words: Tea, High-Speed Countercurrent Chromatography, Theaflavins

CLC Number:

JIANG He-yuan, CHENG Qi-kun, DU Qi-zhen. Effects of Modifying pH on the Separation of Theaflavins by HSCCC[J]. Journal of Tea Science, 2003, 23(s1): 88-91.

References 10

[1]	江和源,程启坤,杜琪珍,等. 红茶中的茶黄素[J]. 中国茶叶,1998;20(3):18–20.
[2]	程启坤. 红茶中的茶黄素[J]. 国外农学──茶叶,1983, (1):1–14.
[3]	陈宗懋. 茶对人体的生理调节机能[J]. 茶叶文摘,1994, (1):1–8; (2):1–9.
[4]	杜琪珍,江和源. 茶色素的药理及其功效[J]. 中国茶叶,1997, 19(5):42–43.
[5]	Collier P D, Bryce T, Mallows R, et al. The Theaflavins of Black Tea[J]. Tetrahedon, 1973, 29: 125-142.
[6]	江和源,程启坤,杜琪珍. 高速逆流色谱在茶黄素分离上的应用[J]. 茶叶科学,2000,20(1):40~44.
[7]	Ma Ying, Yoichiro Ito.Separations of Basic Amino Acid Benyl Esters by pH-Zone-Refining Countercurrent Chromatography[J]. J Chromatog, 1994, 678: 233-240.
[8]	Yoichiro Ito, Ma Ying. pH-Zone Refining Countercurrent Chromatography: a Displacement Mode Applied to Separation of Dinitrophenyl Amino Acids[J]. J Chromatog, 1994, 672: 101-108.
[9]	Yoichiro Ito. pH-Peak-Focusing and pH-Zone-Refining Countercurrent Chromatography[J]. High-Speed Countercurrent Chromatography., Edited by Yoichiro Ito and Walter D. Conway. 1996, 121-175.
[10]	YoichiroIto, Ma Ying. pH-Zone-Refining Countercurrent Chromatography[J]. J Chromatog, 1996, 753: 1-36.

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Effects of Modifying pH on the Separation of Theaflavins by HSCCC

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