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Journal of Tea Science ›› 2006, Vol. 26 ›› Issue (3): 171-176.doi: 10.13305/j.cnki.jts.2006.03.003

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Genetic Mapping of First Generation of Backcross in Tea by RAPD and ISSR Markers

HUANG Fu-ping1, 2, 3, LIANG Yue-rong1, LU Jian-lian1, CHEN Rong-bing2   

  1. 1. Zhejiang University Tea Research Institute, Hangzhou 310029, China;
    2. Tea Research Institute, Fujian Academy of Agricultural Sciences, Fu’an 355000, China;
    3. Damin Foodstuff (Zhangzhou) Ltd, Zhangzhou 363000, China
  • Received:2006-02-13 Revised:2006-04-23 Online:2006-09-25 Published:2019-09-11

Abstract: The segregation mode for 94 plants in first generation of back cross (BC1) of tea cultivar Fudingdabaicha were investigated using 14 RAPD primers and 20 ISSR primers. One hundred and seventy four segregation loci was identified. Among the 174 loci, 90 loci or 51.7% were segregated at the expected Mendelian ratio of 1:1, in which 63 belonged to ISSR loci and 27 belonged to RAPD loci, and 36 loci segregated in the ratios of 3:1 or 1:3. Sixty-two of the 126 loci with segregation ratios of 1:1, 3:1 and 1:3 were included in 7 linkage groups through linkage mapping by Mapmaker EXP 3.0 software and the other 64 loci were not included in the 7 linkage groups because of their far genetic distance. This map concluded 46 RAPD markers and 16 ISSR markers, covering distance of 1180.9βcM, with average distance of 20.1βcM. The linkage group LG4 covered the farthest genetic distance with 309.3βcM and group LG6 had the largest number of markers, with 18 loci and its average distance being 15.7βcM.

Key words: Tea(Camellia Sinensis), RAPD, ISSR, BC1, Genetic mapping

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