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Journal of Tea Science ›› 2012, Vol. 32 ›› Issue (2): 135-141.doi: 10.13305/j.cnki.jts.2012.02.013

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Genetic Diversity and Relationship of Tea Germplasm Resources Camellia sinensis var. assamica cv. Jianghua Revealed by ISSR Markers

LI Dan1, LI Duan-sheng2, YANG Chun1, WANG Qing1, LUO Jun-wu1*   

  1. 1. Key Laboratory of Tea Science of Ministry of Education, Hunan Agricultural University, Changsha 410128, China;
    2. Agriculture Bureau of Jianghua Yao Autonomous County Hunan Province, Jianghua Yao Autonomous County 425509, China
  • Received:2011-07-19 Revised:2012-01-18 Online:2012-04-15 Published:2019-09-05

Abstract: The 10 pairs of ISSR primers were used to analyze the genetic polymorphism and genetic relationship of 70 Camellia sinensis var. assamica cv. Jianghua individuals. Totally 174 bands were generated with 10 ISSR primers, of which 166 (95.40%) were polymorphic, which indicated that the genetic diversity of Jianghua population was relatively high. The mean Nei´s gene diversity (H) and Shannon´s information index (I) of the tested tea germplasm were 0.382 and 0.558 respectively, indicating a rather high genetic diversity existed between the germplasm resources. The genetic similarity coefficient ranged from 0.44 to 0.84, averaging 0.63. UPGMA cluster analysis showed that all the tested individuals could be classified into 6 groups including 5 complex groups and 1 simple group. The genetic relationship of all 70 Jianghua individuals was revealed by the ISSR dendrogram, which provide the basis for the utilization and protection of this local landrace population.

Key words: Camellia sinensis var. assamica cv. Jianghua, ISSR, genetic polymorphism, genetic relationship

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