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Journal of Tea Science ›› 2010, Vol. 30 ›› Issue (2): 141-148.doi: 10.13305/j.cnki.jts.2010.02.011

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Analysis of Genetic Diversity and Relationship of Oolong Tea Varieties and Strains Using SSR Markers

DUAN Yun-shang1, CHENG Hao2,*, JIANG Yan-hua2, WANG Li-yuan2, ZENG Jian-ming2, LI Xing-hui1,*   

  1. 1. Tea Science Research Institute, Nanjing Agricultural University, Nanjing 210095, China;
    2. National Center for Tea Improvement, Tea Research Institute Chinese Academy of Agricultural Sciences, Hangzhou 310008, China
  • Received:2009-06-25 Revised:2009-12-17 Published:2019-09-10

Abstract: The genetic diversity and relationship of 46 Oolong tea varieties and strains from Guangdong, Fujian and Taiwan were analyzed by 40 pairs of SSR markers. Totally 179 alleles and 329 genotypes were amplified by 40 SSR markers, with the average of 4.48 and 8.23. The average polymorphism information content (PIC) was 0.52.Gene diversity (H), observed heterozygosity (Ho) and genetic diversity were 0.57, 0.40 and 0.59, respectively. Genetic diversity of Oolong tea varieties and stains from Guangdong was higher than that from Fujian and Taiwan. All the tested varieties and strains were clustered according to its geographic source and breeding source using both UPGMA and PCA method. The genetic diversity of tested varieties and strains gained by cross breeding was lower than that of samples bred through other methods. In conclusion, the genetic polymorphism of 46 Oolong tea varieties was relatively abundant and the relationship among them would be of great help to effectively select the breeding parents.

Key words: Oolong tea, varieties and strains, SSR, genetic diversity, genetic relationship

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