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Journal of Tea Science ›› 2015, Vol. 35 ›› Issue (4): 329-335.doi: 10.13305/j.cnki.jts.2015.04.004

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Analysis of Genetic Diversity and Relationship of Qimen Population of Tea Plants Based on EST-SSR Markers

LI Saijun, LEI Yu, DUAN Jihua, LUO Yi, HUANG Feiyi, DONG Lijuan*   

  1. Hunan Tea Research Institute, Hunan Branch of National Center for Tea Improvement, Changsha 410125, China
  • Received:2015-03-13 Revised:2015-04-07 Online:2015-10-15 Published:2019-08-26

Abstract: The 24 pairs of EST-SSR primers were used to analyze the genetic polymorphism and genetic relationship of 66 Qimen plants and ‘Anhui 1’. It was showed that the level of genetic diversity within Qimen population was high, with 69 alleles amplified by the 24 EST-SSR primers averaged 2.88. The polymorphism information content (PIC) was ranged from 0.18 to 0.85, with a mean 0.47. The observed heterozygosity (Ho) varied from 0.09 to 0.96, and the expected heterozygosity (He) varied from 0.23 to 0.76, with average of 0.41 and 0.50, respectively. The Shannon index of the Qimen plant population ranged from 0.39 to 1.50, with an average of 0.89. The 66 Qimen plant individuals and cultivar ‘Anhui 1’ were classified into 6 groups based on the UPGMA method with the similarity coefficient at 0.50, and the group I contained 44 Qimen individuals and ‘Anhui 1’, accounted for the 67.16% of the total, which is considered to be core group of the Qimen population.

Key words: Qimen population, EST-SSR, genetic polymorphism, genetic relationship

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