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Journal of Tea Science ›› 2010, Vol. 30 ›› Issue (2): 149-156.doi: 10.13305/j.cnki.jts.2010.02.012

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Genetic Diversity and Relationship of Tea Germplasm Resources ‘Huangjincha’ (Camellia sinensis)Revealed by ISSR Markers

NING Jing1,2, LI Jian-quan1, DONG Li-juan1, YANG Yang1, ZHANG Shu-guang1,*, TAN Zheng-chu1, ZHONG Xing-gang1, ZHU Qi2, PENG Ji-guang1, HUANG Jian-an2   

  1. 1. Hunan Tea Research Institute, Hunan Academy of Agricultural Sciences, Changsha 410125, China;
    2. Key Laboratory of Tea Science of Ministry of Education, Hunan Agricultural University, Changsha 410128, China
  • Received:2009-10-09 Revised:2009-12-20 Published:2019-09-10

Abstract: Genetic diversity and relationship of 110 strains from ‘Huangjincha’ population were analyzed by inter-simple sequence repeat (ISSR) markers. A total of 205 bands were detected by using 18 ISSR primers, of which 187 were polymorphic, and the total percentage of polymorphic bands was 91.22%. Results showed that abundant diversities existed among tea strains studied. The average value of effective number of alleles, Nei’s gene diversity and Shannon’s information index of 110 tea trains were 1.51, 0.30, 0.45 respectively, which indicated that the genetic diversity of ‘Huangjincha’ population was relatively high. The Jaccard similarity coefficient of 110 tea strains ranged from 0.22 to 1.00, and the average value was 0.55. Total of 110 tea strains were divided into seven groups by UPGMA according to the average value of similarity coefficient, and ISSR dendrogram was established, which revealed the genetic relationship between different strains. All the results offer us some supports from molecular level to protect ‘Huangjincha’ population, select fine varieties and crossing parents from them, etc.

Key words: Huangjincha (C. sinensis), ISSR, genetic diversity, genetic relationship

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