茶树发根中茶氨酸和儿茶素类含量的分析

doi:10.13305/j.cnki.jts.2006.04.014

Abstract

Abstract: Hairy roots induced by Agrobacterium rhizogenes wild strain 15834 were propagated on LG₀ medium and half MS medium containing 1βmg/L IBA to promote rapid growth. Although rapid growth was obtained in half MS media containing 1βmg/L IBA, high content of theanine (23.12βmg/g fresh weight) was only detected in one hairy root clone cultured on LG₀ medium and higher than the non-transformed root (18.77βmg/g fresh weight). High content of aspartic acid, glutamic acid, glutamine, histidine and arginine were also detected in this hairy root clones. Medium constitute also affected the contents of amino acid in hairy root clone, the content of theanine were reduced in two hairy root clones when they were cultivated on half MS with 1βmg/L IBA. Low content of simple catechins, catechin (C) and epicatechin (EC), were also detected in hairy roots.

Key words: Camellia sinensis, hairy root culture, theanine, catechins, HPLC

CLC Number:

S571.1

ZHANG Guang-hui, LIANG Yue-rong, LU Jian-liang, DONG Jun-jie. Analysis of Theanine and Catechins in Hairy Roots of Camellia sinensis[J]. Journal of Tea Science, 2006, 26(4): 305-309.

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Analysis of Theanine and Catechins in Hairy Roots of Camellia sinensis

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