茶饮料中微量色素物质的HPLC分析方法研究

doi:10.13305/j.cnki.jts.2007.04.014

Abstract

Abstract: Study on HPLC method for analyzing of trace pigments in ready-to-drink tea was conducted by using different eluants with acetonitrile as mobile phase. Result showed that gradient elution was suitable for analyzing by using acetonitrile/acetic acid/water in ratio of 3/0.5/96.5(v/v/v) as mobile phase A, and using acetonitrile /methanol /chloroform in ratio of 75/15/10(v/v/v) as mobile phase B. The optimized elution time program could be described that phase B increased gradiently from 80% to 100% in early 20 minutes, then remained 100% for 15 minutes. The main pigments, i.e. neoxanthin, violaxanthin, lutein, chlorophyll b, chlorophyll a, phaeophytin b, β-Carotene and phaeophytin a, could be separated upto baseline under that condition. Verification test showed that the optimized HPLC method with good repeatability and low detection limits was suitable for measurement of trace pigments in ready-to-drink tea.

Key words: Camellia sinensis, ready-to-drink tea, HPLC, pigments

CLC Number:

PAN Shun-shun, LU Jian-liang, YANG Xiao-li, ZHENG Xin-qiang, DU Ying-ying, Devijat B, LIANG Yue-rong*. Study on HPLC Method for the Analysis of Trace Pigments in Ready-to-drink Tea[J]. Journal of Tea Science, 2007, 27(4): 343-348.

References

[1] 方元超, 梅丛笑, 杨朝伟. 茶汤色、香、味的变化机理及提高茶汤品质的方法[J]. 冷饮与速冻食品工业, 2000(4): 19~21.
[2] 林亲录, 刘冠民. 绿茶饮料保色的探讨[J]. 食品科学, 1994(9): 18~20.
[3] 赵良. 绿茶饮料的护色和稳定性研究[J]. 食品研究与开发, 1998, l9(1): 29~31.
[4] 罗龙新. 液体茶储藏期的品质变化[J]. 中国茶叶, 1998(4): 11~l3.
[5] 方元超, 梅丛笑. 绿茶饮料的护色技术[J]. 茶叶机械杂志, 1999(4): 1~3.
[6] 梁月荣, 陆建良, 马辉. 罐装绿茶饮料防褐变研究[J]. 浙江农业大学学报, 1999, 25(3): 260~262.
[7] 梁月荣. 茶饮料特性及工艺研究[J]. 粮油加工与食品机械, 2001(7): 12~14.
[8] 李小满. 茶饮料关键控制点及其处理的研究[J]. 广州食品工业科技, 2001, 17(1): 80~83.
[9] 高新蕾. 绿茶饮料防褐变的研究[J]. 饮料工业, 2002, 5(2): 22~26.
[10] 杨晓萍, 郭大勇, 周家刚. 绿茶饮料汤色的稳定性[J]. 食品工业, 2003(2): 19~20.
[11] Delia BRA.A guide to carotenoid analysis in foods[M]. Washington, DC, ILSI Press, 2001: 15~16.
[12] Mieko K, Delia BRA.A scheme for obtaining standards and HPLC quantification of leafy vegetable carotenoids[J]. Food Chemistry, 2002, 78: 389~398.
[13] 孙庆磊, 梁月荣, 陆建良, 等. 不同浸提方法对茶汤品质的影响[J]. 茶叶, 2005, 31(2): 9l~94.
[14] Walker JS, Keely BJ.Distribution and significance of chlorophyll derivatives and oxidation products during the spring phytoplankton bloom in the Celtic Sea April 2002[J]. Organic Geochemistry, 2004, 35: 1289~1298.
[15] Mantoura RFC, Llewellyn CA.The rapid determination of algal chlorophyll and carotenoid pigments and their breakdown products in natural waters by reverse-phase high performance liquid chromatography[J]. Anal Chim Acta, 1983, 151: 297~314.
[16] 潘顺顺. 绿茶鲜汁饮料色素物质组成及其护绿措施研究[M]. 浙江大学硕士学位论文, 2007.

Study on HPLC Method for the Analysis of Trace Pigments in Ready-to-drink Tea

PDF (PC)

Knowledge

Cited

Abstract

Cite this article

share this article

References

Related Articles 15

Metrics

Comments

Recommended 10

[1]	WANG Liubin, HUANG Liyun, TENG Cuiqin, WU Liyun, CHENG Hao, YU Cuiping, WANG Liyuan. Genetic and Phylogenetic Analysis for Germplasm Resources of Camellia sinensis from Wuzhou City [J]. Journal of Tea Science, 2022, 42(5): 601-609.
[2]	ZHOU Hanchen, YANG Jihong, XU Yujie, WU Qiong, LEI Pandeng. Phylogenetic Analysis of NUDX1 Gene Involved in Geraniol Biosynthesis [J]. Journal of Tea Science, 2022, 42(5): 638-648.
[3]	CHEN Yuhong, GAO Ying, HAN Zhen, YIN Junfeng. Analysis of the Saponin Contents and Composition in Tea Seeds of Different Germplasms [J]. Journal of Tea Science, 2022, 42(5): 705-716.
[4]	XING Anqi, WU Zichen, XU Xiaohan, SUN Yi, WANG Genmei, WANG Yuhua. Research Advances of Fluoride Accumulation Mechanisms in Tea Plants (Camellia sinensis) [J]. Journal of Tea Science, 2022, 42(3): 301-315.
[5]	WANG Tao, WANG Yiqing, QI Siyu, ZHOU Zhe, CHEN Zhidan, SUN Weijiang. Identification and Transcriptional Regulation of CLH Gene Family and Expression Analysis in Albino Tea Plants (Camellia sinensis) [J]. Journal of Tea Science, 2022, 42(3): 331-346.
[6]	LIU Fuhao, FAN Yangen, WANG Yu, MENG Fanyue, ZHANG Lixia. Screening and Identification of Chaperone CsHIPP26.1 Chelating Ions in Tea Cultivar ‘Huangjinya’ [J]. Journal of Tea Science, 2022, 42(2): 179-186.
[7]	WANG Pengjie, YANG Jiangfan, ZHANG Xingtan, YE Naixing. Research Advance of Tea Plant Genome and Sequencing Technologies [J]. Journal of Tea Science, 2021, 41(6): 743-752.
[8]	ZHOU Hanchen, LEI Pandeng. The Functional Identification of Two Alternative Splicing Transcripts of CsNES [J]. Journal of Tea Science, 2021, 41(6): 753-760.
[9]	YAN Minghui, LIU Ke, WANG Man, LYU Ying, ZHANG Qian. Complete Chloroplast Genome of Camellia sinensis cv. Xinyang 10 and Its Phylogenetic Evolution [J]. Journal of Tea Science, 2021, 41(6): 777-788.
[10]	LIN Xinying, WANG Pengjie, CHEN Xuejin, GUO Yongchun, GU Mengya, ZHENG Yucheng, YE Naixing. Identification of LOX Gene Family in Camellia sinensis and Expression Analysis in the Process of White Tea Withering [J]. Journal of Tea Science, 2021, 41(4): 482-496.
[11]	WANG Yanding, WANG Huan, LI Nana, WANG Lu, HAO Xinyuan, WANG Yuchun, DING Changqing, YANG Yajun, WANG Xinchao, QIAN Wenjun. Identification and Expression Analysis of Glucose-6-hosphate Dehydrogenase Gene (CsG6PDHs) in Camellia sinensis [J]. Journal of Tea Science, 2021, 41(4): 497-510.
[12]	CHEN Siwen, KANG Rui, GUO Zhiyuan, ZHOU Qiongqiong, FENG Jiancan. Cloning and Expression Analysis of CsCML16 in Tea Plants (Camellia sinensis) under Low Temperature Stress [J]. Journal of Tea Science, 2021, 41(3): 315-326.
[13]	ZHAO Yiqing, LIU Zhengjun, ZHANG Tianxin, ZHAO Yanting, XIAO Bin, GAO Yuefang. Cloning of CsCHLI Gene and Its Expression Analysis in Different Albino Tea Cultivars (Camellia sinensis) [J]. Journal of Tea Science, 2021, 41(3): 327-336.
[14]	GUO Lingling, ZHANG Fen, CHENG Hao, WEI Kang, RUAN Li, WU Liyun, WANG Liyuan. Molecular Cloning and Expression Analysis of CsAAPs Gene Subfamily in Camellia Sinensis [J]. Journal of Tea Science, 2020, 40(4): 454-464.
[15]	WANG Minghan, DING Ding, ZHANG Chenyu, GAO Xizhi, CHEN Jianjiao, TANG Han, SHEN Chengwen. Effects of Drought Stress on Growth and Chlorophyll Fluorescence Characteristics of Tea Seedlings [J]. Journal of Tea Science, 2020, 40(4): 478-491.