茶树花发育相关的一个钙依赖蛋白激酶基因的克隆与表达分析

doi:10.13305/j.cnki.jts.2009.1.008

Abstract

Abstract: To have an insight into the comprehensive molecular characteristics relating to the flower development mechanisms of tea plant [Camellia sinensis (L.)O.Kuntze], cDNA-AFLP (Amplified Fragment Length Polymorphism) was performed within two tea plant cultivars, Longjin43 and Daye Wulong. A cDNA fragment encoding a calcium-dependent protein kinase gene was isolated and identified. The complete cDNA sequence was cloned by RACE (rapid amplification of cDNA ends), named TCK(Camellia sinensis calcium-dependent protein kinase)gene. GenBank accession number is EU732607. To identify differential expression of TCK gene, RT-PCR experiments were performed in six samples including leaves, small flower buds and big flower buds of Longjing 43 and Daye Wulong. Results showed that the expect band exists in the big flower buds, no band in leaves and small flower buds of these two cultivars. It hinted that TCK might play a key role during flower development of tea plant.

Key words: Camellia sinensis, flower development, calcium-dependent protein kinase gene, cloning, differential expression

CLC Number:

S571.1
Q523

CHEN Xuan, TANG Cha-qin, ZOU Zhong-wei, YANG Yi-yang, FANG Wan-ping, WANG Yu-hua, LI Xing-hui. Molecular Cloning and Expression of a Flowering-related Calcium-dependent Protein Kinase Gene in Tea Plant [Camellia sinensis (L.)O.Kuntze][J]. Journal of Tea Science, 2009, 29(1): 47-52.

References

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Molecular Cloning and Expression of a Flowering-related Calcium-dependent Protein Kinase Gene in Tea Plant [Camellia sinensis (L.)O.Kuntze]

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