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Journal of Tea Science ›› 2010, Vol. 30 ›› Issue (6): 435-439.doi: 10.13305/j.cnki.jts.2010.06.005

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Determination of Monosaccharides and Disaccharides in Tea by High Performance Liquid Chromatography- Evaporative Light Scattering Detector

YUAN Yong, HUANG Jian-an, LI Yin-hua, CHEN Jin-hua, YUAN Ling, LIU Zhong-hua*   

  1. Key Lab of Tea Science of Ministry of Education, Hunan Agricultural University, National Research Center of Engineering & Technology for Utilization of Functional Ingredients from Botanical, Changsha 410128, China
  • Received:2010-03-19 Revised:2010-05-06 Published:2019-09-11

Abstract: A method for determination of monosaccharide and disaccharide in tea by HPLC-ELSD was developed. Shodex NH2P-50-4E (4.6 mm×250 mm, 5 μm) column was used at 20℃, acetonitrile and water used as mobile phase, for gradient elusion with the flow rate of 1.0 mL/min. The ratio of organic solvent was controlled by a gradient elution procedure. The procedure was as follows: 85% acetonitrile in 0~14 min, 70%~80% acetonitrile 14~16 min, 70% acetonitrile 16~28 min, then 70%~80% acetonitrile 28~32 min. At last, the ratio of acetonitrile increased to 85%. With the use of evaporative light-scattering detector, the nebulization chamber temperature was 30℃, the drift tube temperature was 70℃ and nitrogen pressure was 310.275 Pa. The calibration curves of rhamnose, arabinose, xylose, fructose, mannose, glucose, sucrose and maltose showed good linearity in the range of 3.202~184.828 μg. The recoveries of fortified 8 kinds of sugar were 90.97%~105.07%, limit of detention was 6.976~1 376.297 ng. Results indicated that the method was simple, rapid and could effectively separate the target component.

Key words: HPLC, ELSD, tea, monosaccharides, disaccharides

CLC Number: