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Journal of Tea Science ›› 2012, Vol. 32 ›› Issue (2): 95-99.doi: 10.13305/j.cnki.jts.2012.02.002

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Determination of Nitrate and Nitrite in Tea by Ion Chromatography

CAI Hui-mei1, HOU Ru-yan1, GAO Zhu2, PENG Chuan-yi1, WAN Xiao-chun1*   

  1. 1. Anhui Agricultural University, Key Laboratory of Tea Biochemistry & Biotechnology, Ministry of Education & Ministry of Agriculture, Hefei 230036, China;
    2. China national Center for Quality Supervision and Test of Agricultural-avocation Processed Food, Hefei 230001, China
  • Received:2011-05-09 Revised:2011-12-08 Online:2012-04-15 Published:2019-09-05

Abstract: A method for simultaneous determination of nitrite and nitrate in tea was established. Tea samples were pre-treated by ultrasonic extraction, PVPP and solid-phase extraction cartridge clean up, and the resulting supernatants were injected into a high-capacity anion exchange column with suppressed conductivity detection. The results showed that the recovery nitrate was 88.6%~107.5% and nitrite was 89.3%~101.2%. The relative standard deviations (RSD) were 1.67% and 1.13% respectively. It was concluded that the advantages of the method are simple operation, fast and high accuracy.

Key words: ion chromatography, tea, nitrate, nitrite

CLC Number: