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Journal of Tea Science ›› 2013, Vol. 33 ›› Issue (2): 147-154.doi: 10.13305/j.cnki.jts.2013.02.013

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Bacterial Abundance of Tea Garden Soils and Its Influencing Factors

HAN Wen-yan1, WANG Wan-meng2, GUO Yun3, YANG Ming-zhen1, JIA Zhong-jun2,*   

  1. 1. Tea Research Institute, Chinese Academy of Agricultural Sciences, Hangzhou 310008, China;
    2. Institute of Soil Science, Chinese Academy of Sciences, Nanjing 210008, China;
    3. College of Geography Science, Nanjing Normal University in Jiangsu, Nanjing 210046, China
  • Received:2012-08-13 Revised:2012-10-08 Online:2013-04-30 Published:2019-09-04

Abstract: Bacterial abundances in tea garden and their adjacent forest and vegetable soils were investigated by real-time quantitative PCR (qPCR) as well as the factors that may affect the population size of bacterial communities. Soil DNA was extracted by using Griffiths’ method and bacterial abundance was determined by quantifying the copy number of 16S rRNA genes. The results showed that the bacterial abundance of tea garden soils ranged from 0.01×108 to 20.32×108 16βS rRNA gene copies/g (gram dry weight soil) with an average of 3.70×108 16βS rRNA gene copies/g, being similar with that in the forest soil, but far below that in the vegetable soil. The bacterial abundance in the tea garden soils was significantly and positively correlated with the soil pH and microbial biomass C (P<0.001) respectively, but significantly and negatively correlated with N application rate and age of tea plantation (P<0.01) respectively. There was no significant correlation between bacterial abundance and total organic C and total N in soil. Multiple regression analysis further indicated that bacterial abundance was affected most significantly by soil pH, followed by age of tea stand and annual N application rate. The results of this study suggested that soil amelioration such as raising soil pH and reducing the high rates of nitrogen application could be of great help for maintaining bacterial abundance and microbial diversity in tea garden soils.

Key words: tea, soil, Griffiths method, bacterial abundance, DNA, pH, N application rate

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