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Journal of Tea Science ›› 2014, Vol. 34 ›› Issue (2): 180-187.doi: 10.13305/j.cnki.jts.2014.02.014

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Study of SNaPshot Detect SNP Markers in Tea Plant

ZHANG Chengcai1, TAN Liqiang1,2, WANG Liyuan1, WEI Kang1, CHENG Hao1,*   

  1. 1. Tea Research Institute, Chinese Academy of Agriculture Science, National Center for Tea Improvement, Hangzhou 310008, China;
    2. College of Horticulture, Sichuan Agricultural University, Yaan 614025, China
  • Received:2013-07-01 Revised:2013-08-12 Online:2014-04-15 Published:2019-09-03

Abstract: In order to increase the genotyping efficiency of tea SNPs and promote the application of SNPs in tea genetic breeding investigation, the feasibility of SNaPshot in SNPs genotyping of tea plant was investigated. Ten SNPs were selected from previous experiment as target SNPs. Then, these SNPs were detected in different tea cultivars by SNaPshot technology. Six among 10 SNPs were successful detected, with an accuracy of 60%. The polymorphism diversity of these SNPs was also analyzed. The value of NA was 2, He ranged from 0.37 to 0.52, Ho ranged from 0.32 to 0.74, PIC ranged from 0.36 to 0.50. All these markers were shown coincide between `Longjing43` and its repeated test. The markers reported here will be useful for tea genetic linkage map construction and genetic diversity study. The SNaPshot technology will promote the genetic study and accelerate the breeding process in tea plant.

Key words: tea plant, SNPs, SNaPshot, genetic diversity, genetic linkage map

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