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Journal of Tea Science ›› 2014, Vol. 34 ›› Issue (3): 297-306.doi: 10.13305/j.cnki.jts.2014.03.013

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Isolation of CsRAV2 Transcription Factor Gene of Tea Plant and its Expression Analysis

WU Zhijun, LI Xinghui, FANG Wanping, ZHOU Lin, ZHAO Zhen, ZHUANG Jing*   

  1. Tea Research Institute, College of Horticulture, Nanjing Agricultural University, Nanjing 210095, China
  • Received:2013-08-13 Revised:2013-11-01 Online:2014-06-15 Published:2019-09-03

Abstract: The RAV transcription factor, one subfamily of AP2/ERF family transcription factor, includes several genes that encode proteins involved in the development and regulation of abiotic/biotic resistance in higher plant. The CsRAV2 genes, which encoding to the RAV transcription factor, were cloned from tea plant (Camellia sinensis) cultivars ‘Anjibaicha’ and ‘Yingshuang’ by PCR and RT-PCR using DNA and cDNA as template, respectively. Then, nucleic acid and deduced amino acid sequence, phylogenetic tree, and molecular modeling were predicted and analyzed. The lengths of CsRAV2 genes from the two tea plant cultivars were 1 089 bp, encoding 362 amino acids. No intron was found in the CsRAV2 gene. The transcription factor of CsRAV2 contained two distinct DNA domains mainly found in higher plants RAV family factors, one AP2 domain together with one B3 domain. The CsRAV2 were hydrophilic protein. The protein of CsRAV2 from tea plant and AtRAV from Arabidopsis had similar three-dimension structure. Quantitative real-time PCR analysis of the expression profiles showed that the CsRAV2 gene was tissue-specific expressed in the two tea plant cultivars. The highest expression levels of the CsRAV2 gene were found in the root. The CsRAV2 gene was induced by high temperature, low temperature, PEG and high-salinity treatment, respectively. There were differences profiles between different teacultivars.

Key words: Camellia sinensis, transcription factor, RAV family factors, phylogenetic analysis, three-dimensional structure, expression analysis

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