Gene Cloning and Expression Analysis of Actin in Tea Plant Root

LI Yuanhua; LU Jianliang; FAN Fangyuan; SHI Yutao

doi:10.13305/j.cnki.jts.2015.04.005

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Journal of Tea Science ›› 2015, Vol. 35 ›› Issue (4) : 336-346. DOI: 10.13305/j.cnki.jts.2015.04.005

Gene Cloning and Expression Analysis of Actin in Tea Plant Root

LI Yuanhua¹, LU Jianliang², FAN Fangyuan², SHI Yutao¹

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Abstract

By using SSH, we analyzed differences in gene expression of root from Fuding white tea infected by VA mycorrhiza and obtained diversity sequences. The sequence alighment showed that the down-regulated expression sequence possibly contained 10 unknown genes and the up-regulated expression sequence possible contained 5 known genes. The Actin genic full-length sequence was obtained by using RACE. The length of Actin gene was 1β606βbp (GenBank Accession No. KJ946252), with 1β131βbp ORF (1^st-1β131^st), the sequence encoded 377 amino acid. Bioinformatics indicated that the Actin protein’s molecular weight was about 30.69 kD, IEP was 5.27, located in subcellular fraction area like cell nucleus. The study also showed Actin expressed no obvious difference in different cultivars and it responded weak to non-biological stress.

Key words

actin / expression / gene clone / tea plant

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LI Yuanhua, LU Jianliang, FAN Fangyuan, SHI Yutao. Gene Cloning and Expression Analysis of Actin in Tea Plant Root[J]. Journal of Tea Science. 2015, 35(4): 336-346 https://doi.org/10.13305/j.cnki.jts.2015.04.005

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