Acid-assisted Improvement of Dispersive Solid Phase Extraction for Rapid Detection of Pesticide Residues in Tea

WU Zhenghao; ZHENG Qinqin; HAO Zhenxia; WANG Chen; CHEN Hongping; LU Chengyin

doi:10.13305/j.cnki.jts.2023.03.008

PDF(774 KB)

Journal of Tea Science ›› 2023, Vol. 43 ›› Issue (3) : 389-398. DOI: 10.13305/j.cnki.jts.2023.03.008

Research Paper

Acid-assisted Improvement of Dispersive Solid Phase Extraction for Rapid Detection of Pesticide Residues in Tea

WU Zhenghao^1,4, ZHENG Qinqin^1,2,3,*, HAO Zhenxia^1,2,3, WANG Chen^1,2,3, CHEN Hongping^1,2,3, LU Chengyin^1,2,3,*

Author information +

History +

Abstract

The false-positive results encountered in the rapid detection of pesticide residues in tea samples are primarily caused by matrix interference. However, limited by the physical and chemical properties of tea matrix components (e.g., caffeine), the development of pretreatments suffers major dilemmas in matrix removal effectiveness. In current work, a novel pretreatment method for tea samples was proposed by developing specific removal procedures based on the molecular structure characteristics of the main matrix components. On the basis of conventional pretreatment for tea samples, the novel method introduced acid solutions and polyvinylpolypyrrolidone adsorbents, which reduced the matrix effect of tea sample detection significantly with a high matrix removal rate of more than 99.99%. Under the assistance of the current pretreatment, the rapid detection of methomyl and carbofuran residues in tea samples was successfully achieved accurately. The limits of detection for carbofuran and methomyl in tea were 0.57 mg·kg^-1 and 0.20 mg·kg^-1, respectively.

Key words

matrix interference / pesticide residue / pretreatment / rapid detection / tea

Cite this article

EndNote

Ris (Procite)

Bibtex

Download Citations

WU Zhenghao, ZHENG Qinqin, HAO Zhenxia, WANG Chen, CHEN Hongping, LU Chengyin. Acid-assisted Improvement of Dispersive Solid Phase Extraction for Rapid Detection of Pesticide Residues in Tea[J]. Journal of Tea Science. 2023, 43(3): 389-398 https://doi.org/10.13305/j.cnki.jts.2023.03.008

References

[1] Zhang L, Cao Q Q, Granato D, et al.Association between chemistry and taste of tea: a review[J]. Trends in Food Science & Technology, 2020, 101: 139-149.
[2] Hu S P, Zhao M, Mao Q Q, et al.Rapid one-step cleanup method to minimize matrix effects for residue analysis of alkaline pesticides in tea using liquid chromatography-high resolution mass spectrometry[J]. Food Chemistry, 2019, 299: 125146. doi: 10.1016/j.foodchem.2019.125146.
[3] 金莉莉, 郝振霞, 高贯威, 等. 超高效液相色谱-静电场轨道阱高分辨质谱法测定茶叶中21种农药残留量[J]. 茶叶科学, 2018, 38(6): 595-605.
Jin L L, Hao Z X, Gao G W, et al.Determination of 21 pesticide residues in tea by ultra performance liquid chromatography orbitrap high resolution mass spectrometry[J]. Journal of Tea Science, 2018, 38(6): 595-605.
[4] Fabiani C, Murray A, Corradi J, et al.A novel pharmacological activity of caffeine in the cholinergic system[J]. Neuropharmacology, 2018, 135: 464-473.
[5] Zhang S, Zhu Q, Chen J Y, et al.The pharmacological activity of epigallocatechin-3-gallate (EGCG) on Alzheimer's disease animal model: a systematic review[J]. Phytomedicine, 2020, 79: 153316. doi: 10.1016/j.phymed.2020.153316.
[6] Mazumder M K, Choudhury S.Tea polyphenols as multi-target therapeutics for Alzheimer’s disease: an in silico study[J]. Medical Hypotheses, 2019, 125: 94-99.
[7] Chen W, Cai F, Wu Q.Prediction, evaluation, confirmation, and elimination of matrix effects for lateral flow test strip based rapid and on-site detection of aflatoxin B1 in tea soups[J]. Food Chemistry, 2020, 328: 127081. doi: 10.1016/j.foodchem.2020.127081.
[8] 金莉莉. 基于微流控纸芯片技术的有机磷和氨基甲酸酯类农药残留快速检测研究[D]. 北京: 中国农业科学院, 2019.
Jin L L.Rapid detection of organophosphorus and carbamate pesticide residues based on microfluidic paper-based analytical devices[D]. Beijing: Chinese Academy of Agricultural Sciences, 2019.
[9] Brahmbhatt H A, Surtees A, Tierney C, et al.Effect of polymerisation by microwave on the physical properties of molecularly imprinted polymers (MIPs) specific for caffeine[J]. Polymer Chemistry, 2020, 11(36): 5778-5789.
[10] Teixeira L S, Silva C F, De Oliveira H L, et al. Microextraction by packed molecularly imprinted polymer to selectively determine caffeine in soft and energy drinks[J]. Microchemical Journal, 2020, 158: 105252. doi: 10.1016/j.microc.2020.105252.
[11] Ferreira S S, Alves A, Filipe-Ribeiro L, et al.Holistic and sustainable approach for recycling and valorization of polyvinylpolypyrrolidone used in wine fining[J]. ACS Sustainable Chemistry & Engineering, 2018, 6(11): 14599-14606.
[12] Laborde B, Moine-Ledoux V, Richard T, et al.PVPP-polyphenol complexes: a molecular approach[J]. Journal of Agricultural and Food Chemistry, 2006, 54(12): 4383-4389.
[13] Liang S, Granato D, Zou C, et al.Processing technologies for manufacturing tea beverages: from traditional to advanced hybrid processes[J]. Trends in Food Science & Technology, 2021, 118: 431-446.
[14] 李云飞, 戴前颖, 夏涛, 等. 添加PVPP对绿茶汤色的影响及作用机理的研究[J]. 食品工业科技, 2012, 33(22): 127-132.
Li Y F, Dai Q Y, Xia T, et al.Research of effect and mechanism of infusion color of green tea by adding PVPP[J]. Science and Technology of Food Industry, 2012, 33(22): 127-132.
[15] Sharma E, Joshi R, Gulati A.L-Theanine: an astounding sui generis integrant in tea[J]. Food Chemistry, 2018, 242: 601-610.
[16] Kramer D N, Gamson R M, Miller F.A study of the physical and chemical properties of the esters of indophenols I. Preparation[J]. The Journal of Organic Chemistry, 1959, 24(11): 1742-1747.
[17] Jin L L, Hao Z X, Zheng Q Q, et al.A facile microfluidic paper-based analytical device for acetylcholinesterase inhibition assay utilizing organic solvent extraction in rapid detection of pesticide residues in food[J]. Analytica Chimica Acta, 2020, 1100: 215-224.
[18] 林伟东, 孙威江, 郭义红, 等. 茶叶中茶氨酸的研究与利用[J]. 食品研究与开发, 2016, 37(20): 201-206.
Lin W F, Sun W J, Guo Y H, et al.The research and utilization of theanine in tea[J]. Food Research and Development, 2016, 37(20): 201-206.
[19] 葛艳. 夏秋绿茶中茶氨酸的提取分离纯化及对肠道微生态的影响[D]. 南京: 南京农业大学, 2013.
Ge Y.Extraction, isolation and purification of theanine from summer-autumn green tea and its influence on intestinal microecology[D]. Nanjing: Nanjing Agricultural University, 2013.
[20] 徐悦. PVPP在茶饮料中应用的研究[D]. 杭州: 浙江大学, 2017.
Xu Y.Study on application of PVPP in tea beverage[D]. Hangzhou: Zhejiang University, 2017.
[21] Cao Y Y, Qi X R, Yan H S.Selective adsorption of tannins over small polyphenols on cross-linked polyacrylamide hydrogel beads and their regeneration with hot water[J]. Reactive and Functional Polymers, 2020, 146: 104398. doi: 10.1016/j.reactfunctpolym.2019.104398.