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Journal of Tea Science ›› 2013, Vol. 33 ›› Issue (6): 526-531.doi: 10.13305/j.cnki.jts.2013.06.013

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Genetic Diversity of Tea Germplasm Resource ‘Huangjincha’ (Camellia sinensis) Revealed by AFLP Analysis

WU Yang, DENG Ting-ting, LI Juan, LI Yin-hua, LIU Shuo-qian, WANG Ying-zi, HUANG Jian-an*, LIU Zhong-hua*   

  1. National Research Center of Engineering Technology for Utilization of Botanical Functional Ingredients,Key Lab of Tea Science of Ministry of Education of Hunan Agricultural University, Changsha 410128, China
  • Received:2013-03-31 Revised:2013-05-07 Online:2013-12-30 Published:2019-09-04

Abstract: 111 tea strains from ‘Huangjincha’ population were analyzed by AFLP-sliver staining protocol for detecting the genetic diversity and relationship. AFLP fingerprinting of 111 samples with primer combinations E37M32, E41M33 and E41M42 revealed a total number of unambiguous 229 bands, of which 186 were polymeric and the polymorphism frequency was 81.22%. The results showed that abundant diversities existed among samples of genomic DNA. The mean of effective number of alleles, Nei′s gene diversity and Shannon′s information index of 111 samples were 1.42±0.35, 0.25±0.18, 0.38±0.25 respectively. As analyzed by NTSYSpc2.1, the similarity coefficient of 111 samples ranged from 0.65 to 0.99, and the mean was 0.76. These tea strains were divided into 8 groups by UPGMA based on the mean of similarity coefficient, and AFLP dendrogram was established. In conclusion, the results provide the basis for the protection and utilization of ‘Huangjincha’ population from molecular level.

Key words: Huangjincha (Camellia sinensis), AFLP, genetic diversity

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