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Journal of Tea Science ›› 2023, Vol. 43 ›› Issue (2): 147-158.doi: 10.13305/j.cnki.jts.2023.02.009

• Research Paper •     Next Articles

Genetic Diversity Analysis of Tea Genetic Resources in Sanjiang, Guangxi

MENG Rongjun1, CHEN Liang2, XU Yuan1, LIN Wei3, ZHOU Qiwei3, XIE Yilin3, LAI Dingqing4,*, LAI Jiaye3,*   

  1. 1. College of Forestry, Guangxi University, Nanning 530004, China;
    2. Tea Research Institute, Chinese Academy of Agricultural Sciences, Hangzhou 310008, China;
    3. Institute of Agriculture and Animal Husbandry Development, Guangxi University, Nanning 530004, China;
    4. Sanjiang County Duoyelou Tea Industry Co., Ltd., Sanjiang 545500, China
  • Received:2022-12-15 Revised:2023-01-19 Online:2023-04-15 Published:2023-05-05

Abstract: In order to clarify the genetic diversity of tea genetic resources in Sanjiang, Guangxi, two marker techniques, simple inter-repeat sequence amplification (ISSR) and correlated sequence amplification polymorphism (SRAP) were used to analyze the genetic diversity of 72 local tea genetic resources and five introduced cultivars in Sanjiang region. The results of both marker techniques show high levels of genetic diversity (H=0.30, I=0.44), high genetic differentiation between populations (Gst=0.056) and gene flow (Nm=8.64). The genetic variation observed within populations was much higher than that between populations. The cluster analysis shows that most of the tea genetic resources in Sanjiang were clustered into one group and the introduced cultivars were clustered into the other, which was consistent with the results of PCoA. This study provided evidence at the molecular level for the protection and utilization of tea germplasmin Sanjiang.

Key words: Camellia sinensis, molecular marker, genetic diversity, population structure

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