茶树根系HGMR基因克隆及表达分析

doi:10.13305/j.cnki.jts.2014.06.020

Abstract

Abstract: By using SSH, the differences in gene expression of root system from Fuding Dabai tea infected by VA mycorrhiza were analyzed and diversity sequences were obtained. The sequence alignment showed that the mentioned sequence contain 3-hydroxy-3-methylglutaryl coenzyme A reductase named HMGR. HGMR full-length sequence was obtained by using RACE. The length of HGMR gene is 2 420 bp, with 1 773 bp ORF (163^rd-1935^th), and the sequence encoded 590 amino acids. Bioinformatics indicated that the HGMR protein’s molecular weight is about 63.5 kD, IEP is 6.8, which located in mitochondria or endoplasmic reticulum membrane. The study also showed expression degree of HGMR is distinct in different cultivars, while it responded obviously to biological and non-biological stress.

Key words: tea plant, 3-hydroxy-3-methylglutaryl coenzyme a reductase, gene clone, expression

CLC Number:

S571.1
Q52

LI Yuanhua, LU Jianliang, FAN Fangyuan, SHI Yutao. Gene Cloning and Expression Analysis of HMGR in Tea Plant Roots[J]. Journal of Tea Science, 2014, 34(6): 583-590.

References 18

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Gene Cloning and Expression Analysis of HMGR in Tea Plant Roots

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