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Journal of Tea Science ›› 2009, Vol. 29 ›› Issue (3): 243-250.doi: 10.13305/j.cnki.jts.2009.3.011

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Genetic Diversity and Structure of Tea Germplasm Originated from Region of North Yangtze River Based on EST-SSR Markers

YAO Ming-zhe1,2, LIU Zhen2, CHEN Liang2,*, WANG Xin-chao2, MA Chun-lei2, LIANG Yue-rong1,*   

  1. 1. College of Agriculture and Biological Technology, Zhejiang University, Hangzhou 310029, China;
    2. National Center for Tea Improvement, Tea Research Institute Chinese Academy of Agricultural Sciences, Hangzhou 310008, China
  • Received:2009-02-17 Revised:2009-03-16 Online:2009-06-15 Published:2019-09-06

Abstract: The 25 pairs of EST-SSR primers were used to analyze the genetic diversity, genetic structure and genetic relationship of 45 tea accessions originated from region of north Yangtze River. Totally 83 alleles were identified, on an average of 3.3 alleles each pair of primers. The mean of polymorphism information content (PIC) was 0.61. The observed heterozygosity (Ho) was averagely higher than the expected heterozygosity (He). Among 45 tea accessions the average numbers of the observed alleles were 4.2, and the number of effective alleles 2.8. The observed heterozygosity, gene diversity and Shannon information index were 0.73, 0.61 and 1.11, respectively. The low genetic difference (Gst=0.2) and high geneflow (Nm=3.9) of tea populations from Hubei, Anhui and Shaanxi were found. Analysis of molecular variance (AMOVA) showed that variance components inter-population and intra-population were 4.03% and 95.97% respectively. The paired similarity coefficients of 45 accessions were from 0.32 to 0.89. No clearly regional partition among 45 accessions was found from the dendrogram. The high level of genetic similarity was demonstrated among tea population of Hubei, Anhui and Shaanxi province, and Shaanxi population showed relatively distant relationship comparing to population of Hubei and Anhui.

Key words: tea, EST-SSR, genetic diversity, genetic structure, Camellia sinensis

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