Abstract: In order to purify theaflavins industrially, three macroporous adsorptive resins with different polarity such as NKA-9,AB-8 and ADS-7 were investigated and six factors as the type of resin, pH value, sample filling rate, effluent concentration of eliminating impurity, effluent concentration of sample and effluent volume of sample were determined. The result showsed that NKA-9 possessed a better purify effect than that of AB-8 and ADS-7, and the optimum condition is pH value of 3, sample filling rate of 2BV/h, 15% ethanol of eliminating impurity and 3BV 90% ethanol to elute. The purity of theaflavins can reach to 44.1% under this condition. But the purification condition had some difference between total mixture and each monomer.

Key words: macroporous adsorptive resins, theaflavins, purify