Analysis of Genetic Diversity and Population Structure of 32 Tea Landraces in China

LI Changle; GE Yue; YAN Meilin; LI Hui; LIN Qingqing; WANG Pu; ZHAO Hua; WANG Mingle; WANG Yu; GUO Fei; NI Dejiang

doi:10.13305/j.cnki.jts.20210804.002

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Journal of Tea Science ›› 2021, Vol. 41 ›› Issue (5) : 619-630. DOI: 10.13305/j.cnki.jts.20210804.002

Research Paper

Analysis of Genetic Diversity and Population Structure of 32 Tea Landraces in China

LI Changle, GE Yue, YAN Meilin, LI Hui, LIN Qingqing, WANG Pu, ZHAO Hua, WANG Mingle, WANG Yu, GUO Fei^*, NI Dejiang

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Abstract

Tea plant populations are natural populations of tea plants. They are cultivated in specific production areas and contain individuals with different economic and biological traits. There are great differences among individuals and there are many types. They are important materials for studying the evolutionary relationship and breeding of tea plants. In this study, 30 pairs of SSR primers distributed on 15 linkage groups were selected to analyze the genetic diversity and population structure of 32 tea plant populations from 12 provinces, in order to provide a reference for the selection of tea breeding parents and the inference of evolution route. In this study, a total of 149 alleles were obtained with an average of 5.96 for each SSR marker. The average polymorphism information content of primers was 0.660. Shannon's diversity index of 32 tea populations ranged from 0.691 to 1.089, with an average of 0.954. The observed heterozygosity ranged from 0.253 to 0.633, with an average of 0.510. The expected heterozygosity ranged from 0.430 to 0.653, with an average of 0.590. The average genetic differentiation coefficient (Fst) of tea plant population was 0.205, indicating a high level of genetic differentiation. The results of clustering based on Nei's genetic distance and population structure analysis were consistent. The germplasm to be tested was divided into 4 major types, with obvious regional distribution.

Key words

evolutionary route / genetic diversity / population structure / SSR molecular marker / tea landraces

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LI Changle, GE Yue, YAN Meilin, LI Hui, LIN Qingqing, WANG Pu, ZHAO Hua, WANG Mingle, WANG Yu, GUO Fei, NI Dejiang. Analysis of Genetic Diversity and Population Structure of 32 Tea Landraces in China[J]. Journal of Tea Science. 2021, 41(5): 619-630 https://doi.org/10.13305/j.cnki.jts.20210804.002

References

[1] 谢文钢, 李晓松, 李伟, 等. 四川地方中小叶茶树资源的表型遗传多样性[J]. 浙江农业学报, 2019, 31(9): 1405-1415.
Xie W G, Li X S, Li W, et al.Genetic diversity of “Small & Medium Leaf” tea resources in Sichuan Province based on phenotypic characteristics[J]. Acta Agriculturae Zhejiangensis, 2019, 31(9): 1405-1415.
[2] 黄晓敏. 闽南茶区不同乌龙茶品种叶片显微结构比较研究[D]. 福州: 福建农林大学, 2010.
Huang X M.Comparative research on leaf tissues for different oolong tea cultivars in southern tea region of Fujian Province [D]. Fuzhou: Fujian Agriculture and Forestry University, 2010.
[3] 许燕. 6个特色茶树品种(系)光合特性及叶绿体超微结构研究[D]. 成都: 四川农业大学, 2016.
Xu Y.Studies on photosynthetic traits and chloroplast ultrastructure of 6 tea characteristic varieties (lines) [D]. Chengdu: Sichuan Agriculture University, 2016.
[4] Martinez L E, Cavagnaro P F, Masuell R W, et al.SSR-based assessment of genetic diversity in south American Vitis vinifera varieties[J]. Plant Science, 2006, 170(6): 1036-1044.
[5] Yao M Z, Chen L, Liang Y R.Genetic diversity among tea cultivars from China, Japan and Kenya revealed by ISSR markers and its implication for parental selection in tea breeding programmes[J]. Plant Breeding, 2008, 127(2): 166-172.
[6] Yao M Z, Ma C L, Qiao T T, et al.Diversity distribution and population structure of tea germplasms in China revealed by EST-SSR markers[J]. Tree Genetics & Genome, 2012, 8(1): 205-220.
[7] Zhao L P, Liu Z, Chen L, et al.Generation and characterization of 24 novel EST derived microsatellites from tea plant (Camellia sinensis) and cross-species amplification in its closely related species and varieties[J]. Conservation Genetics, 2008, 9(5): 1327-1331.
[8] Bandyopadhyay T.Molecular marker technology in genetic improvement of tea[J]. International Journal of Plant Breeding and Genetics, 2011, 5(1): 23-33.
[9] Fang W P, Meinhardt L W, Tan H W, et al.Varietal identification of tea (Camellia sinensis) using nanofluidic array of single nucleotide polymorphism (SNP) markers[J]. Horticulture Research, 2014, 1(1): 14035. doi: 10.1038/hortres.2014.35.
[10] Hu C Y, Tsai Y Z, Lin S F.Development of STS and CAPS markers for variety identification and genetic diversity analysis of tea germplasm in Taiwan[J]. Botanical Studies, 2014, 55(1): 1-15.
[11] 李婧慧, 金彦龙, 乔艳清, 等. 新疆150份陆地棉品种DNA指纹图谱构建与遗传多样性分析[J/OL]. 分子植物育种: 2020[2021-03-02]. http://kns.cnki.net/kcms/detail/46.
1068.S.20201217.1805.004.html.Li J H, Jin Y L, Qiao Y Q, et al. Construction of DNA fingerprint and genetic diversity of 150 upland cotton varieties in Xinjiang [J/OL]. Molecular Plant Breeding: 2020[2021-03-02]. http://kns.cnki.net/kcms/detail/46.1068.S.20201217.1805.004.html.
[12] 姜慧, 潘根, 常丽, 等. 亚麻分子标记辅助育种研究进展[J/OL]. 植物遗传资源学报:2020[2021-03-02]. https://doi.org/10.13430/j.cnki.jpgr.20201124001.
Jiang H, Pan G, Chang L, et al. Advances in molecular marker assisted breeding of flax [J/OL]. Journal of Plant Genetic Resources:2020[2021-03-02]. https://doi.org/10.13430/j.cnki.jpgr. 20201124001.
[13] 黄丹娟. 我国茶树优良品种遗传多样性及指纹图谱构建[D]. 北京: 中国农业科学院, 2016.
Huang D J.Genetic diversity and fingerprinting of Chinese improved tea cultivars [D]. Beijing: Chinese Academy of Agricultural Sciences, 2016.
[14] 姜晓辉, 方开星, 陈栋, 等. 基于EST-SSR毛细管电泳荧光标记技术分析广东2个历史名茶群体遗传多样性[J]. 热带作物学报, 2018, 39(1): 46-54.
Jiang X H, Fang K X, Chen D, et al.Genetic diversity and relationship of two historical famous Camellia sinensis groups in Guangdong by capillary electrophoresis detection with fluorescent EST-SSR marker[J]. Chinese Journal of Tropical Crops, 2018, 39(1): 46-54.
[15] 杨军, 王让剑, 孔祥瑞, 等. 94个茶树种质资源遗传多样性与亲缘关系分析[J]. 茶叶学报, 2018, 59(4): 186-192.
Yang J, Wang R J, Kong X R, et al.Genetic diversity and relationship of 94 tea germplasm[J]. Acta Tea Sinica, 2018, 59(4): 186-192.
[16] 姚明哲, 马春雷, 陈亮, 等. 川､渝地方茶树品种的遗传多样性和群体结构[J]. 茶叶科学, 2012, 32(5): 419-425.
Yao M Z, Ma C L, Chen L, et al.Genetic diversity and population structure of local tea landraces in Sichuan and Chongqing revealed by EST-SSR markers[J]. Journal of Tea Science, 2012, 32(5): 419-425.
[17] Zhao D, Yang J, Yang S, et al.Genetic diversity and domestication origin of tea plant Camellia taliensis (Theaceae) as revealed by microsatellite markers[J]. BMC Plant Biology, 2014, 14(1): 14. doi: 10.1186/1471-2229-14-14.
[18] Khawla G A, Jasmine K J, Rose L A.Chloroplast genome analysis of box-ironbark Eucalyptus[J]. Molecular Phylogenetics and Evolution, 2019, 136: 76-86.
[19] 朱小虎. 新疆密叶杨群体遗传多样性及遗传结构研究[D]. 北京: 北京林业大学, 2019.
Zhu X H.Study on genetic diversity and genetic structure of Populus talassica Kom. population in Xinjiang [D]. Beijing: Beijing Forestry University, 2019.
[20] Hieter P A.Clustered arrangement of immunoglobulin lambda constant region genes in man[J]. Nature, 1981, 294: 536-540.
[21] 刘爽. 中国樟树群体遗传结构研究[D]. 南昌: 江西农业大学, 2019.
Liu S.Population genetic structure of Cinnamomum camphora in China [D]. Nanchang: Jiangxi Agricultural University, 2019.
[22] 黄燕, 朱振东, 段灿星, 等. 灰葡萄孢蚕豆分离物的遗传多样性[J]. 中国农业科学, 2014, 47(12): 2335-2347.
Huang Y, Zhu Z D, Duan C X, et al.Genetic diversity of Botrytis cinerea isolates from broad bean[J]. Scientia Agricultura Sinica, 2014, 47(12): 2335-2347.
[23] 乔婷婷. 茶树资源遗传多样性及其表型性状关联EST-SSR位点的初步鉴定[D]. 北京: 中国农业科学院, 2010.
Qiao T T.Genetic diversity of tea (Camellia sinensis (L.) O. Kuntze) and association analysis of phenotypic traits with EST-SSR markers [D]. Beijing: Chinese Academy of Agricultural Sciences, 2010.
[24] 汤存伟, 余雄, 刘武军, 等. 新疆13个绵羊群体遗传多样性及遗传分化的研究[J]. 家畜生态学报, 2011, 32(1): 13-19.
Tang C W, Yu X, Liu W J, et al.Study on genetic diversity and genetic differentiation of 13 sheep populations in Xinjiang Uygur Autonomous Region[J]. Journal of Domestic Animal Ecology, 2011, 32(1): 13-19.
[25] Wright S.Evolution and the genetics of populations. 4: variability within and among natural populations [M]. Chicago: The University of Chicago Press, 1978.
[26] 刘振, 成杨, 杨培迪, 等. 基于nSSR和cpDNA序列的城步峒茶群体遗传多样性和结构研究[J]. 茶叶科学, 2020, 40(2): 250-258.
Liu Z, Cheng Y, Yang P D, et al.Genetic diversity and structure of Chengbudong tea population revealed by nSSR and cpDNA markers[J]. Journal of Tea Science, 2020, 40(2): 250-258.
[27] 王铠元, 孙克祥, 卢瑞甫. 滇西地区大地构造演化[J]. 青藏高原地质文集, 1983(4): 187-199.
Wang K Y, Sun K X, Lu R P.Tectonic evolution in western Yunnan[J]. Collected Works of Qinghai-Tibet Plateau Geology, 1983(4): 187-199.
[28] 明庆忠. 纵向岭谷北部三江并流区河谷地貌发育及其环境效应研究[D]. 兰州: 兰州大学, 2006.
Ming Q Z.The landform development and environmental effects in the north of Longitudinal Range-Gorge Region (LRGR) [D]. Lanzhou: Lanzhou University, 2006.
[29] Schultz M.Phylogeography’s past, present, and future: 10 years after Avise, 2000[J]. Molecular Phylogenetics and Evolution, 2010, 54(1): 291-301.
[30] 姚明哲, 刘振, 陈亮, 等. 利用EST-SSR分析江北茶区茶树资源的遗传多样性和遗传结构[J]. 茶叶科学, 2009, 29(3): 243-250.
Yao M Z, Liu Z, Chen L, et al.Genetic diversity and structure of tea germplasm originated from region of north Yangtze River based on EST-SSR markers[J]. Journal of Tea Science, 2009, 29(3): 243-250.
[31] 姜燕华. 我国茶树地方品种遗传多样性及人为选择影响的研究[D]. 北京: 中国农业科学院, 2010.
Jiang Y H.Studies on the genetic diversity of traditional tea germplasm in China as well as the influence of artificial selection [D]. Beijing: Chinese Academy of Agricultural Sciences, 2010.
[32] 丁洲, 李烨昕, 袁艺, 等. 安徽茶区优良群体种的表型性状和遗传多样性分析[J]. 茶叶科学, 2018, 38(2): 155-161.
Ding Z, Li Y X, Yuan Y, et al.Phenotypic traits and genetic diversity of elite tea population in Anhui (China)[J]. Journal of Tea Science, 2018, 38(2): 155-161.
[33] 余书平, 徐礼羿, 吴荣梅, 等. 浙江开化县茶树种质资源的遗传多样性及亲缘关系分析[J]. 茶叶科学, 2020, 40(3): 341-351.
Yu S P, Xu L Y, Wu R M, et al.Genetic and phylogenetic analysis for resources of Camellia Sinensis from Kaihua County in Zhejiang Province[J]. Journal of Tea Science, 2020, 40(3): 341-351.