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Journal of Tea Science ›› 2023, Vol. 43 ›› Issue (2): 287-296.doi: 10.13305/j.cnki.jts.20230418.001

• Research Paper • Previous Articles    

Inhibitory Effect and Mechanism of Theaflavin (TF1) on Hepatoma Carcinoma Bel-7402 Cells

WANG Zhuan1, WEI Wujun2, REN Zhenzhen2, HE Zhilong1, FAN Yuchun3, ZHOU Jie3, GUO Guiyi4, JIANG Lihe1,2,3,*   

  1. 1. College of Light Industry and Food Engineering, Guangxi University, Nanning 530004, China;
    2. School of Basic Medical Sciences, Youjiang Medical University for Nationalities, Baise 533000, China;
    3. Medical College, Guangxi University, Nanning 530004, China;
    4. Henan Key Laboratory of Tea Comprehensive Utilization in South Henan Xinyang Agriculture and Forestry University, Xinyang 464000, China
  • Received:2022-07-26 Revised:2022-11-18 Online:2023-04-15 Published:2023-05-05

Abstract: To explore the inhibitory effect and mechanism of theaflavin (TF1), human hepatoma carcinoma Bel-7402 cells were treated with different concentrations of TF1. Cell viability was detected by CCK8 and cell proliferation was detected by colony formation assay. Cell migration was detected by cell wound healing experiment and transwell chamber assay. Cell apoptosis was detected by flow cytometry. Apoptosis-related proteins (Bax, Bcl-2, PARP), migration related proteins (E-cad, N-cad, Vimentin, MMP9) and signaling pathway related proteins (TGF-β, Smad3, p-smad3) were detected by western blot. The results show that different concentrations of TF1 could inhibit the proliferation and migration of Bel-7402 cells and promote their apoptosis in a dose-dependent manner. The higher the dose, the stronger the inhibition effect (P<0.05). Compared with the control group, the expression levels of Bax and E-cad proteins in the experimental group were higher, while the expression levels of Bcl-2, PARP, N-cad, Vimentin, MMP9, TGF-β, Smad3 and p-smad3 were lower (P<0.05). In conclusion, TF1 could inhibit the proliferation and migration of Bel-7402 cells and promote their apoptosis through TGF-β signaling pathway.

Key words: theaflavin, human hepatoma carcinoma cell, mechanism research

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