Isolation, Screening and Identification of A Strain of Trichoderma Antagonizing Tea Anthracnose

ZHAO Xingli; ZHANG Jinfeng; ZHOU Yufeng; ZHAO Dailin; ZHANG Li; ZHOU Luona; TAO Gang

doi:10.13305/j.cnki.jts.2019.04.008

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Journal of Tea Science ›› 2019, Vol. 39 ›› Issue (4) : 431-439. DOI: 10.13305/j.cnki.jts.2019.04.008

Isolation, Screening and Identification of A Strain of Trichoderma Antagonizing Tea Anthracnose

ZHAO Xingli¹, ZHANG Jinfeng², ZHOU Yufeng^1,*, ZHAO Dailin³, ZHANG Li⁴, ZHOU Luona¹, TAO Gang^3,*

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Abstract

To obtain antagonistic Trichoderma for biocontrol of tea anthracnose, the twenty-three Trichoderma strains were isolated from Camellia sinensis rhizosphere-soil by taking gradient dilution. Among them, the strain LS17110205 against tea anthracnose was screened by using dual-culture and inhibition zone assay. The results show that the inhibitory rate of LS17110205 against tea anthracnose was up to 76.96% and a large number of white hyphae and green spores were produced on the colony of tea anthracnose, which caused the tea anthracnose colony to shrink and became dark. The fermentation liquid of LS17110205 was effective against pathogen hyphae, and the inhibitory rate was 70.08%. The results show that the fermentation of the strain LS17110205 caused the tea anthracnose mycelia to shrink on the surface by scanning electron microscope analysis. LS17110205 was identified as Trichoderma asperelloides on the basis of the morphological characteristics and phylogenetic tree. The results provided theoretical basis in biocontrol of tea anthracnose.

Key words

Trichoderma spp / identification / inhibitory rate / rhizosphere-soil / tea anthracnose

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ZHAO Xingli, ZHANG Jinfeng, ZHOU Yufeng, ZHAO Dailin, ZHANG Li, ZHOU Luona, TAO Gang. Isolation, Screening and Identification of A Strain of Trichoderma Antagonizing Tea Anthracnose[J]. Journal of Tea Science. 2019, 39(4): 431-439 https://doi.org/10.13305/j.cnki.jts.2019.04.008

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