茶树新梢不同叶片中β-葡萄糖苷酶和β-樱草糖苷酶基因表达的实时定量PCR分析

doi:10.13305/j.cnki.jts.2006.01.002

茶叶科学 ›› 2006, Vol. 26 ›› Issue (1): 11-16.doi: 10.13305/j.cnki.jts.2006.01.002

茶树新梢不同叶片中β-葡萄糖苷酶和β-樱草糖苷酶基因表达的实时定量PCR分析

赵丽萍,陈亮^*,王新超,姚明哲

中国农业科学院茶叶研究所/农业部茶叶化学工程重点实验室茶树资源遗传改良与分子生物学实验室, 浙江杭州 310008

收稿日期:2005-09-06 修回日期:2005-12-31 出版日期:2006-03-25 发布日期:2019-09-10
通讯作者: *陈亮,liangchen@mail.tricaas.com
作者简介:赵丽萍(1979-- ),黑龙江肇东人,硕士,从事茶树分子生物学研究。
基金资助:
国家自然科学基金（30070486）、浙江省重点科技项目（2004C22033）、“十五”国家科技攻关计划（2004-07）等

Quantitative Detection of β-glucosidase, β-primeverosidase Gene Expressions in Different Leaves of Tea Plantby Real-Time PCR Analysis

ZHAO Li-ping, CHEN Liang^*, WANG Xin-chao, YAO Ming-zhe

Lab for Tea Germplasm, Genetic Improvement and Molecular Biology, Tea Research Institute Chinese Academy of Agricultural Sciences; Key Laboratory of Tea Chemical Engineering, the Ministry of Agriculture, Hangzhou 310008, China

Received:2005-09-06 Revised:2005-12-31 Online:2006-03-25 Published:2019-09-10

摘要/Abstract

摘要： 在建立茶树基因表达绝对定量实时PCR检测方法后,检测了龙井43新梢不同部位叶片中的β-葡萄糖苷酶和β-樱草糖苷酶基因的表达情况,结果表明β-葡萄糖苷酶在一芽五叶新梢的第四叶中表达活性最高,为2.86E+08拷贝/µl,第四叶>第三叶>第五叶>第二叶>一芽一叶; 而β-樱草糖苷酶基因在一芽一叶中最高,为4.31E+06拷贝/µl,一芽一叶>第二叶>第三叶>第四叶>第五叶。与茶叶香气密切相关两个基因在茶树不同部位的叶片中表达量和表达类型存在明显差异。本实验建立的实时荧光定量PCR可有效地用于茶树基因表达的定量分析。

关键词: 茶树, β-葡萄糖苷酶;, β-樱草糖苷酶;, 实时定量PCR

Abstract: β-glucosidase and β-primeverosidase are considered the most important genes related to made tea aroma. An absolute quantification method for gene expression in tea plant using real-time PCR analysis was established and, the expression of β-glucosidase and β-primeverosidase genes in different leaves of Longjing 43 young shoot were determined using this method. The results indicate that the fourth leaf of the ‘five and a bud’ shoot has the highest copy number of β-glucosidase mRNA, which is 2.86E+08 copies/μl, the 4^th leaf>the 3^rd leaf>the 5^th leaf>the 2^nd leaf>one and a bud. And the highest β-primeverosidase expression is in the one and a bud, corresponding to 4.31E+06 copies/μl, one and a bud>the 2^nd leaf>the 3^rd>the 4^th leaf> the 5^th leaf. The expression intensity and pattern of these genes, which are closely related to tea aroma, are different in the young shoot. The results illuminate that the new developed method could be effectively used to detect gene expression quantitatively in tea plant.

Key words: tea plant, β-glucosidase, β-primeverosidase, real-time PCR

中图分类号:

S571.1
Q523

赵丽萍, 陈亮, 王新超, 姚明哲. 茶树新梢不同叶片中β-葡萄糖苷酶和β-樱草糖苷酶基因表达的实时定量PCR分析[J]. 茶叶科学, 2006, 26(1): 11-16. doi: 10.13305/j.cnki.jts.2006.01.002.

ZHAO Li-ping, CHEN Liang, WANG Xin-chao, YAO Ming-zhe. Quantitative Detection of β-glucosidase, β-primeverosidase Gene Expressions in Different Leaves of Tea Plantby Real-Time PCR Analysis[J]. Journal of Tea Science, 2006, 26(1): 11-16. doi: 10.13305/j.cnki.jts.2006.01.002.

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茶树新梢不同叶片中β-葡萄糖苷酶和β-樱草糖苷酶基因表达的实时定量PCR分析

Quantitative Detection of β-glucosidase, β-primeverosidase Gene Expressions in Different Leaves of Tea Plantby Real-Time PCR Analysis

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