叶绿素仪（SPAD）在茶树氮素营养诊断中的适用性研究

doi:10.13305/j.cnki.jts.2008.04.007

茶叶科学 ›› 2008, Vol. 28 ›› Issue (4): 301-308.doi: 10.13305/j.cnki.jts.2008.04.007

叶绿素仪（SPAD）在茶树氮素营养诊断中的适用性研究

杨亦扬^1,2, 马立锋², 石元值², 阮建云^2,*, 黎星辉^1,*

1. 南京农业大学茶叶研究所,江苏南京 210095;
2. 农业部茶叶化学工程重点实验室,中国农业科学院茶叶研究所,浙江杭州,310008

收稿日期:2007-10-08 出版日期:2008-08-15 发布日期:2019-09-12
通讯作者: *,jruan@mail.tricaas.com
作者简介:杨亦扬（1983– ）,女,江苏阜宁人,在读博士研究生,研究方向：茶树营养生理。
基金资助:
农业部948项目(2006-G16),浙江省科技项目（2006C22071）和国家“十一五”科技支撑计划(2006BAD06B01)资助

Evaluation of Nitrogen Status in Tea Plants by SPAD

YANG Yi-yang^1,2, MA Li-feng², SHI Yuan-zhi², RUAN Jian-yun^2,*, LI Xing-hui^1,*

1. Tea Research Institute, Nanjing Agriculture University, Nanjing 210095, China;
2. Key Laboratory of Tea Chemical Engineering, the Ministry of Agriculture and Tea Research Institute of Chinese Academy of Agriculture Sciences, Hangzhou 310008, China

Received:2007-10-08 Online:2008-08-15 Published:2019-09-12

摘要/Abstract

摘要： 测定叶片氮素含量是诊断茶树氮素状况的一项重要方法。本文利用盆栽试验,通过不同的供氮水平,研究了利用叶绿素仪（SPAD-502）进行茶树氮素营养快速诊断的适用性。结果表明,茶树新梢产量与氮素用量、成熟叶氮素含量之间呈线性加平台的反应关系,成熟叶的SPAD值与其全氮含量呈显著线性正相关,并与茶树产量存在着比较明显的关系拐点,初步显示SPAD可以较好地表征茶树的氮素营养状况,有可能作为快速诊断方法用于指导茶树施肥,但是还需要通过大田试验进行进一步验证。本文还探讨了测定位点、叶位、天气状况、田间原位或离体测定方式、表面清洗以及样本量等因素对SPAD测试精度的影响。

关键词: 茶树, 叶绿素仪, 氮素营养, 影响因素

Abstract: Determination of nitrogen concentrations in tissues is an important diagnostic means for N status of plants. A pot experiment with tea plants applied with different amounts of N fertilizer was carried out to evaluate the usefulness of SPAD-502 as a diagnostic tool. The results showed that the yield of young shoots and N concentration of mature leaves responded to N application rates with a pattern of an initial linear increase before a platform was reached. There was a significantly positive linear relationship between the SPAD reading and total N concentration of mature leaves. The similar pattern of an initial linear increase followed by a platform was observed for the relationship between SPAD reading and the yield. These results suggested that the N status of tea plants may be characterized by the measurement of SPAD reading of mature leaves and the SPAD is a potentially useful rapid diagnostic tool in N fertilization recommendation. However, this needs further validation from field experiments. Those factors which may influence the precision of SPAD measurement, such as the point of test on a leaf, selection of leaves from twig (position), weather condition, in situ or in vitro analysis, surface cleaning and the number of leaves for a composite sample were also investigated.

Key words: Camellia sinensis L., chlorophyll meter, nitrogen nutrition, affecting factors, SPAD

中图分类号:

杨亦扬, 马立锋, 石元值, 阮建云, 黎星辉. 叶绿素仪（SPAD）在茶树氮素营养诊断中的适用性研究[J]. 茶叶科学, 2008, 28(4): 301-308. doi: 10.13305/j.cnki.jts.2008.04.007.

YANG Yi-yang, MA Li-feng, SHI Yuan-zhi, RUAN Jian-yun, LI Xing-hui. Evaluation of Nitrogen Status in Tea Plants by SPAD[J]. Journal of Tea Science, 2008, 28(4): 301-308. doi: 10.13305/j.cnki.jts.2008.04.007.

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叶绿素仪（SPAD）在茶树氮素营养诊断中的适用性研究

Evaluation of Nitrogen Status in Tea Plants by SPAD

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