叶绿素仪（SPAD）在茶树氮素营养诊断中的适用性研究

杨亦扬; 马立锋; 石元值; 阮建云; 黎星辉

doi:10.13305/j.cnki.jts.2008.04.007

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茶叶科学 ›› 2008, Vol. 28 ›› Issue (4) : 301-308. DOI: 10.13305/j.cnki.jts.2008.04.007

叶绿素仪（SPAD）在茶树氮素营养诊断中的适用性研究

杨亦扬^1,2, 马立锋², 石元值², 阮建云^2,*, 黎星辉^1,*

作者信息 +

Evaluation of Nitrogen Status in Tea Plants by SPAD

YANG Yi-yang^1,2, MA Li-feng², SHI Yuan-zhi², RUAN Jian-yun^2,*, LI Xing-hui^1,*

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文章历史 +

摘要

测定叶片氮素含量是诊断茶树氮素状况的一项重要方法。本文利用盆栽试验,通过不同的供氮水平,研究了利用叶绿素仪（SPAD-502）进行茶树氮素营养快速诊断的适用性。结果表明,茶树新梢产量与氮素用量、成熟叶氮素含量之间呈线性加平台的反应关系,成熟叶的SPAD值与其全氮含量呈显著线性正相关,并与茶树产量存在着比较明显的关系拐点,初步显示SPAD可以较好地表征茶树的氮素营养状况,有可能作为快速诊断方法用于指导茶树施肥,但是还需要通过大田试验进行进一步验证。本文还探讨了测定位点、叶位、天气状况、田间原位或离体测定方式、表面清洗以及样本量等因素对SPAD测试精度的影响。

Abstract

Determination of nitrogen concentrations in tissues is an important diagnostic means for N status of plants. A pot experiment with tea plants applied with different amounts of N fertilizer was carried out to evaluate the usefulness of SPAD-502 as a diagnostic tool. The results showed that the yield of young shoots and N concentration of mature leaves responded to N application rates with a pattern of an initial linear increase before a platform was reached. There was a significantly positive linear relationship between the SPAD reading and total N concentration of mature leaves. The similar pattern of an initial linear increase followed by a platform was observed for the relationship between SPAD reading and the yield. These results suggested that the N status of tea plants may be characterized by the measurement of SPAD reading of mature leaves and the SPAD is a potentially useful rapid diagnostic tool in N fertilization recommendation. However, this needs further validation from field experiments. Those factors which may influence the precision of SPAD measurement, such as the point of test on a leaf, selection of leaves from twig (position), weather condition, in situ or in vitro analysis, surface cleaning and the number of leaves for a composite sample were also investigated.

导出引用

杨亦扬, 马立锋, 石元值, 阮建云, 黎星辉. 叶绿素仪（SPAD）在茶树氮素营养诊断中的适用性研究[J]. 茶叶科学. 2008, 28(4): 301-308 https://doi.org/10.13305/j.cnki.jts.2008.04.007

YANG Yi-yang, MA Li-feng, SHI Yuan-zhi, RUAN Jian-yun, LI Xing-hui. Evaluation of Nitrogen Status in Tea Plants by SPAD[J]. Journal of Tea Science. 2008, 28(4): 301-308 https://doi.org/10.13305/j.cnki.jts.2008.04.007

中图分类号： S571.1 S143.1

参考文献

[1] 李志宏, 张云贵, 刘宏斌, 等. 叶绿素仪在夏玉米氮营养诊断中的应用[J]. 植物营养与肥料学报, 2005, 11(6): 764~768.
[2] 朱新开, 盛海君, 顾晶, 等. 应用SPAD值预测小麦叶片叶绿素和氮含量的初步研究[J]. 麦类作物学报, 2005, 25(2): 46~50.
[3] Minotti P L, Halseth D E, Sieczka J B.Field chlorophyll measurements to asses the nitrogen status of potato varieties[J]. Hort Sci, 1994, 29(12): 1497~150.
[4] Piekielek W P, Fox R H.Use of a chlorophyll meter to predict side-dress nitrogen requirement for maize[J]. Agron J, 1992, 84:59~65.
[5] Singh B, Singh Y, Ladha J K et al. Chlorophyll meter and leaf color cart based nitrogen management for rice and wheat in Northwestern India[J]. Agron J, 2002, 94(4): 821~829.
[6] Turner F T, Jund M F.Chlorophyll meter to predict nitrogen top-dress requirement for semidwart rice[J]. Agron J, 1991, 83: 926~928.
[7] Waskom R M, Westfall D G, Spellman D E, et al. Monitoring nitrogen status of corn with a portable chlorophyll meter[J]. Commun Soil Sci Plant Anal, 1996, 27(3~4): 545~560.
[8] Wood C W, Tracy P W, Reeves D W, et al. Determination of cotton nitrogen status with a hand-held chlorophyll meter[J]. J Plant Nutr, 1992, 15(9): 1435~1448.
[9] Chapman S C, Barreto H J.Using a chlorophyll meter to estimate specific leaf nitrogen of tropical maize during vegetative growth[J]. Agron J, 1997, 89: 557~562.
[10] Campbell R J, Mobley K N, Marini R P, et al. Growing conditions alter relationship between SPAD-501 values and apple leaf chlorophyll[J]. Hort Sci, 1990, 25: 330~331.
[11] Scott X, Changa, Daniel J, et al. Nondestructive and rapid estimation of hardwood foliar nitrogen status using the SPAD-502 chlorophyll meter[J]. Forest Ecol Manag, 2003, 181: 331~338.
[12] 李合生. 植物生理生化实验原理和技术[M]. 北京: 高等教育出版社, 2000.