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茶叶科学 ›› 2009, Vol. 29 ›› Issue (5): 365-371.doi: 10.13305/j.cnki.jts.2009.5.006

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茶树新梢中非酯型儿茶素及其合成酶的变化规律

张宪林1, 高丽萍1,*, 夏涛1,*, 刘亚军2, 高可君1   

  1. 1. 安徽农业大学 农业部茶叶生物化学与生物技术重点实验室;
    2. 安徽农业大学生命科学学院,安徽 合肥 230036
  • 收稿日期:2009-02-07 修回日期:2009-05-25 出版日期:2009-10-15 发布日期:2019-09-09
  • 通讯作者: *
  • 作者简介:张宪林(1982— ),男,河南濮阳人,硕士研究生,从事茶叶生化及天然产物研究。
  • 基金资助:
    国家“973”前期研究计划(2007CB116211)、国家自然科学基金(30771755)和安徽省自然科学基金(090411006)

Study on the Changes of Non-galloylated Catechins and Relative Enzymes in Tea Shoots

ZHANG Xian-lin1, GAO Li-ping1,*, XIA Tao1,*, LIU Ya-jun2, GAO Ke-jun1   

  1. 1. Key Lab of Tea Biochemistry and Biotechnology, Ministry of Agriculture, Anhui Agricultural University;
    2. School of Biology Science, Anhui Agricultural University, Hefei 230036, China
  • Received:2009-02-07 Revised:2009-05-25 Online:2009-10-15 Published:2019-09-09

摘要: 对不同季节茶树新梢不同部位鲜叶中儿茶素组分和非酯型儿茶素合成酶的变化规律进行了研究。结果表明,在茶树新梢上,从芽到第四叶,四种非酯型儿茶素中除了GC外,C、EC和EGC含量均逐步增加;两种非酯型儿茶素合成酶中,DFR/LAR活性呈下降趋势,而ANR在第三叶中达到最高。相关分析进一步表明,不同部位鲜叶中儿茶素的总量变化与DFR/LAR活性变化呈显著正相关,而与ANR变化相关性不大。

关键词: 茶树, 非酯型儿茶素, 不同季节, 生物合成

Abstract: The changes of non-galloylated catechins and relative enzymes as well as the composition of catechins in different parts of tea shoots and in different seasons were investigated in this paper. The results showed that the contents of non-galloylated catechins except GC increased gradually from bud to the fourth leaf. Both of two synthetical enzymes related to non-galloylated cahechins, the activity of DFR/LAR declined gradually, but the activity of ANR was the highest in the third leaf. The result of correlation analysis showed that the contents of total catechins in different tea leaves presented a prominent positive correlation with the activity of DFR/LAR,but not with the activity of ANR.

Key words: tea plant [Camellia sinensis (L.) O. Kuntze], non-galloylated catechins, different seasons, biosynthesis

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