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茶叶科学 ›› 2011, Vol. 31 ›› Issue (5): 458-462.doi: 10.13305/j.cnki.jts.2011.05.015

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铝对茶树根细胞膜透性和根系分泌有机酸的影响

刘腾腾1, 郜红建1,2*, 宛晓春2, 张正竹2   

  1. 1. 安徽农业大学资源与环境学院,安徽 合肥 230036;
    2. 教育部茶叶生物化学与生物技术重点实验室,安徽 合肥 230036
  • 收稿日期:2011-05-09 修回日期:2011-07-04 出版日期:2011-10-15 发布日期:2019-09-09
  • 通讯作者: hjgao@ahau.edu.cn
  • 作者简介:刘腾腾(1984— ),女,山东聊城人,硕士研究生,主要从事茶树生理生化研究。
  • 基金资助:
    教育部科学技术研究重点项目(201097)、安徽省自然科学基金(090411009)、中国博士后基金(20090450800)

Impacts of Aluminum on Root Cell Membrane Permeability and Organic Acids in Root Exudates of Tea Plant

LIU Teng-teng1, GAO Hong-jian1,2*, WAN Xiao-chun2, ZHANG Zheng-zhu2   

  1. 1. Resources and Environment College, Anhui Agricultural University, Hefei 230036, China;
    2. Key Laboratory of Tea Biochemistry & Biotechnology, Ministry of Education, Hefei 230036, China;
  • Received:2011-05-09 Revised:2011-07-04 Online:2011-10-15 Published:2019-09-09

摘要: 采用溶液培养法研究了铝浓度对茶树根细胞膜透性和分泌低分子量有机酸的影响。结果表明,低质量浓度铝(20mg/L)有利于茶树根细胞膜的稳定,缺铝(0mg/L,CK)和高铝(100mg/L)均使根细胞膜透性显著降低。有机酸总量随铝浓度升高呈现先降低后升高的趋势。草酸、苹果酸和柠檬酸是茶树根系分泌的3种主要有机酸,占85%~93%;根系分泌草酸的量与对照相比,低铝和高铝时显著降低了84.7%和34.3%;分泌苹果酸的量与对照相比,低铝时增加了121.0%,高铝时降低了40.9%;铝浓度对柠檬酸的分泌量影响不大。研究结果可为阐明茶树根系的耐铝生理机制提供依据。

关键词: 铝浓度, 茶树, 根细胞膜透性, 有机酸

Abstract: The impacts of aluminum (Al) concentration on root cell membrane permeability and components of organic acids in root exudates of tea plant (Camellia. sinensis L.) were investigated by hydroponics. Results showed that 20mg/L of low Al concentration could enhance the cell membrane stability of tea root, and the membrane permeability was significantly declined with deficiency Al (0mg/L, CK) and high Al (100mg/L) treatments. The total organic acids firstly decreased and then increased with the increase of Al concentrations. About 85%~93% of the total organic acids in root exudates of tea plant were oxalic acid, malic acid and citric acid. Compared with CK, the oxalic acid secretion remarkably reduced 84.7% and 34.3% under the low and high Al concentration treatments. Compared with CK, the malic acid of root exudates increased by 121.1% in low Al concentration, and decreased by 40.9% in high Al concentration. There wasn’t significant influence on citric acid, containing about 3.5~4.5mg/g. This result can provide critical data for elucidating tolerance mechanisms of Al stress on tea root.

Key words: aluminum concentrations, tea plant, root cell membrane permeability, organic acids

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