低咖啡碱茶树遗传群体的咖啡碱含量与分子变异分析

doi:10.13305/j.cnki.jts.2012.03.013

茶叶科学 ›› 2012, Vol. 32 ›› Issue (3): 276-282.doi: 10.13305/j.cnki.jts.2012.03.013

• • 上一篇

低咖啡碱茶树遗传群体的咖啡碱含量与分子变异分析

王雪敏, 姚明哲, 金基强, 马春雷, 陈亮^*

中国农业科学院茶叶研究所茶树资源与改良研究中心,国家茶树改良中心,浙江杭州310008

收稿日期:2012-02-21 修回日期:2012-03-10 出版日期:2012-06-15 发布日期:2019-09-05
通讯作者: liangchen@mail.tricaas.com
作者简介:王雪敏（1988— ）,女,山东滨州人,硕士研究生,主要从事茶树分子遗传学研究。
基金资助:
国家茶叶产业技术体系（CARS-023）、国家自然科学基金（39901159,31170624,31100504）资助

Analysis of Caffeine Content and Molecular Variance of Low-caffeine Tea Plants

WANG Xue-min, YAO Ming-zhe, JIN Ji-qiang, MA Chun-lei, CHEN Liang^*

Tea Research Institute of the Chinese Academy of Agricultural Sciences, National Center for Tea Improvement, Hangzhou 310008, China

Received:2012-02-21 Revised:2012-03-10 Online:2012-06-15 Published:2019-09-05

摘要/Abstract

摘要： 对60个低咖啡碱单株进行咖啡碱含量的HPLC测定和42对EST-SSR引物的分子变异分析。结果表明茶叶中咖啡碱鲜重的变化范围是0.38%~1.08%,低于亲本咖啡碱含量的单株有5份,符合低咖啡碱茶筛选的目标。42对EST-SSR引物在遗传群体中共检测出129个等位基因,每对引物可检测出2~7个等位基因,平均3.86个,平均Shannon-Weaver指数（I）为0.65;标记的多态性信息含量（PIC）平均为0.33,变化范围是0.03~0.68。初步鉴定出3个与咖啡碱变异相关联的分子标记,对低咖啡碱优异基因的筛选及低咖啡碱茶树新品种的选育具有一定的指导意义。

关键词: 茶树, 咖啡碱含量, 分子变异, SSR

Abstract: The caffeine content of 60 individuals of a low-caffeine population of tea plant was analyzed using HPLC and the molecular variance was studied using 42 EST-SSR markers, respectively. The results showed that the caffeine content of fresh weight ranged from 0.38% to 1.08%. Five individuals had lower caffeine content than female parent, and they can be used as breeding materials for further screening low-caffeine tea cultivars. One hundred and twenty-nine alleles were detected, each pair of primers could detect 2 to 7 alleles, an average of 3.86. The average number of Shannon-Weaver index (I) was 0.65. The polymorphism information content (PIC) of EST-SSR markers varied from 0.03 to 0.68, with average of 0.33. Three SSR markers, TM089, TM200 and TM211, related to variation of caffeine content were preliminary identified. It would be of important significance for screening excellent genetic resources and breeding new low-caffeine tea cultivars.

Key words: tea plant, caffeine content, molecular variance, SSR

中图分类号:

S571.1
Q347

王雪敏, 姚明哲, 金基强, 马春雷, 陈亮. 低咖啡碱茶树遗传群体的咖啡碱含量与分子变异分析[J]. 茶叶科学, 2012, 32(3): 276-282. doi: 10.13305/j.cnki.jts.2012.03.013.

WANG Xue-min, YAO Ming-zhe, JIN Ji-qiang, MA Chun-lei, CHEN Liang. Analysis of Caffeine Content and Molecular Variance of Low-caffeine Tea Plants[J]. Journal of Tea Science, 2012, 32(3): 276-282. doi: 10.13305/j.cnki.jts.2012.03.013.

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低咖啡碱茶树遗传群体的咖啡碱含量与分子变异分析

Analysis of Caffeine Content and Molecular Variance of Low-caffeine Tea Plants

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