茶树CsMYB基因启动子的克隆与功能分析

郑世仲; 江胜滔; 刘伟; 陈美霞; 林玉玲; 赖钟雄; 林金科

doi:10.13305/j.cnki.jts.2018.06.004

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茶叶科学 ›› 2018, Vol. 38 ›› Issue (6) : 580-588. DOI: 10.13305/j.cnki.jts.2018.06.004

茶树CsMYB基因启动子的克隆与功能分析

郑世仲^1,2, 江胜滔^1,2, 刘伟^1,2, 陈美霞^1,2, 林玉玲³, 赖钟雄^3,*, 林金科^4,*

作者信息 +

Cloning and Functional Analysis of the CsMYB Promoter In Tea Plant (Camellia sinensis L.)

ZHENG Shizhong^1,2, JIANG Shengtao^1,2, LIU Wei^1,2, CHEN Meixia^1,2, LIN Yuling³, LAI Zhongxiong^3,*, LIN Jinke^4,*

Author information +

文章历史 +

摘要

以茶树新品系“1005”嫩芽为材料,克隆得到CsMYB基因gDNA全长序列1β828βbp,通过染色体步移技术,分离得到CsMYB基因启动子片段1β038βbp,命名为proMYB。生物信息学分析表明,CsMYB gDNA由2个内含子和3个外显子组成,该启动子含有与提高基因转录水平相关的5′UTR Py-rich stretch顺式作用元件、启动子核心元件TATA-box和CAAT-box,还存在有激素响应元件、光响应元件、逆境应答元件以及大量功能未知或功能特异的顺式作用元件,说明proMYB具有诱导型启动子特性,CsMYB基因在茶树植物体中可能参与多种非生物胁迫应答和激素信号传导;通过烟草的瞬时表达结果表明,该启动子能驱动下游基因表达,具有启动子活性。

Abstract

The 1β828βbp full-length gDNA of CsMYB and a 1β038βbp 5′-flanking sequence of CsMYB (named proMYB) were cloned from the shoots of tea cultivar ‘1005’by gDNA cloning and genome-walking method. Bioinformatics analysis showed that the gDNA of CsMYB contained 2 introns and 3 extrons. The cis-acting element analysis showed that the proMYB promoter contained 5′-UTR Py-rich stretch motifs, TATA-box, CAAT-box core elements putative elements responding to hormone, light, stresses and some cis-elements with unknown or specific function, suggesting the inducible character of proMYB. It was also implied that CsMYB might be involved in abiotic stress responses and hormonal signal transduction in tea plant. The transient expression in transformed tobacco showed that the proMYB could drive the expressions of downstream genes.

导出引用

郑世仲, 江胜滔, 刘伟, 陈美霞, 林玉玲, 赖钟雄, 林金科. 茶树CsMYB基因启动子的克隆与功能分析[J]. 茶叶科学. 2018, 38(6): 580-588 https://doi.org/10.13305/j.cnki.jts.2018.06.004

ZHENG Shizhong, JIANG Shengtao, LIU Wei, CHEN Meixia, LIN Yuling, LAI Zhongxiong, LIN Jinke. Cloning and Functional Analysis of the CsMYB Promoter In Tea Plant (Camellia sinensis L.)[J]. Journal of Tea Science. 2018, 38(6): 580-588 https://doi.org/10.13305/j.cnki.jts.2018.06.004

中图分类号： TS272.2 Q52

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