茶树CsMYB基因启动子的克隆与功能分析

doi:10.13305/j.cnki.jts.2018.06.004

茶叶科学 ›› 2018, Vol. 38 ›› Issue (6): 580-588.doi: 10.13305/j.cnki.jts.2018.06.004

茶树CsMYB基因启动子的克隆与功能分析

郑世仲^1,2, 江胜滔^1,2, 刘伟^1,2, 陈美霞^1,2, 林玉玲³, 赖钟雄^3,*, 林金科^4,*

1. 宁德师范学院生命科学学院,福建宁德 352100;
2. 闽东特色生物资源福建省高校工程研究中心,福建宁德 352100;
3. 福建农林大学园艺植物生物工程研究所,福建福州350002;
4. 福建农林大学安溪茶学院,福建福州350002

收稿日期:2018-06-07 修回日期:2018-08-09 出版日期:2018-12-15 发布日期:2019-12-15
通讯作者: *
作者简介:郑世仲,男,博士,主要研究茶树生物技术,Email: zhengshizhong@126.com。
基金资助:
自然科学基金（31170651）、福建省“2011协同创新中心”中国乌龙茶产业协同创新中心专项（闽教科[2015]75号）

Cloning and Functional Analysis of the CsMYB Promoter In Tea Plant (Camellia sinensis L.)

ZHENG Shizhong^1,2, JIANG Shengtao^1,2, LIU Wei^1,2, CHEN Meixia^1,2, LIN Yuling³, LAI Zhongxiong^3,*, LIN Jinke^4,*

1. College of Life Science, Ningde Normal University, Ningde 352100, China;
2. Institute of Fujian Higher Education for Local Bio-resources in Ningde City, Ningde 352100, China;
3. Institute of Horticultural Biotechnology, Fujian Agriculture and Forestry University, Fuzhou 350002, China;
4. Anxi College of Tea Science, Fujian Agriculture and Forestry University, Fuzhou 350002, China

Received:2018-06-07 Revised:2018-08-09 Online:2018-12-15 Published:2019-12-15

摘要/Abstract

摘要： 以茶树新品系“1005”嫩芽为材料,克隆得到CsMYB基因gDNA全长序列1β828βbp,通过染色体步移技术,分离得到CsMYB基因启动子片段1β038βbp,命名为proMYB。生物信息学分析表明,CsMYB gDNA由2个内含子和3个外显子组成,该启动子含有与提高基因转录水平相关的5′UTR Py-rich stretch顺式作用元件、启动子核心元件TATA-box和CAAT-box,还存在有激素响应元件、光响应元件、逆境应答元件以及大量功能未知或功能特异的顺式作用元件,说明proMYB具有诱导型启动子特性,CsMYB基因在茶树植物体中可能参与多种非生物胁迫应答和激素信号传导;通过烟草的瞬时表达结果表明,该启动子能驱动下游基因表达,具有启动子活性。

关键词: 茶树, CsMYB, 启动子, 顺式作用元件, 瞬时表达

Abstract: The 1β828βbp full-length gDNA of CsMYB and a 1β038βbp 5′-flanking sequence of CsMYB (named proMYB) were cloned from the shoots of tea cultivar ‘1005’by gDNA cloning and genome-walking method. Bioinformatics analysis showed that the gDNA of CsMYB contained 2 introns and 3 extrons. The cis-acting element analysis showed that the proMYB promoter contained 5′-UTR Py-rich stretch motifs, TATA-box, CAAT-box core elements putative elements responding to hormone, light, stresses and some cis-elements with unknown or specific function, suggesting the inducible character of proMYB. It was also implied that CsMYB might be involved in abiotic stress responses and hormonal signal transduction in tea plant. The transient expression in transformed tobacco showed that the proMYB could drive the expressions of downstream genes.

Key words: Camellia sinensis, CsMYB, promoter, Cis-acting element, transient expression

中图分类号:

TS272.2
Q52

郑世仲, 江胜滔, 刘伟, 陈美霞, 林玉玲, 赖钟雄, 林金科. 茶树CsMYB基因启动子的克隆与功能分析[J]. 茶叶科学, 2018, 38(6): 580-588. doi: 10.13305/j.cnki.jts.2018.06.004.

ZHENG Shizhong, JIANG Shengtao, LIU Wei, CHEN Meixia, LIN Yuling, LAI Zhongxiong, LIN Jinke. Cloning and Functional Analysis of the CsMYB Promoter In Tea Plant (Camellia sinensis L.)[J]. Journal of Tea Science, 2018, 38(6): 580-588. doi: 10.13305/j.cnki.jts.2018.06.004.

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茶树CsMYB基因启动子的克隆与功能分析

Cloning and Functional Analysis of the CsMYB Promoter In Tea Plant (Camellia sinensis L.)

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