茶树蛋白质提取及双向电泳的改良方法

doi:10.13305/j.cnki.jts.2003.01.004

Abstract

CLC Number:

S571.1
Q51

LIN Jin-ke, ZHENG Jin-gui, YUAN Ming, ZHANG Xue-qin, WANG Feng. The Improvement of Isolation and The Method of Two-Dimensional Electrophoresis of Protein from Tea Plant[J]. Journal of Tea Science, 2003, 23(1): 16-20.

References

[1] 吴庆如,黄开勋,徐辉碧. 双向凝胶电泳技术进展[J]. 生物技术通讯,2002,13(2): 28~31.
[2] 杨何义,应万涛,钱小红. 蛋白质组技术的研究进展[J]. 自然科学进展,2002,12(1):13-19.
[3] Stanley F, Yuji Kohara, David J L, et al. Functional genomics[J]. Proc Natl Acad Sci USA, 1999, 96: 8825-8826.
[4] O Farrell P H. High resolution two dimensional electrophoresis of protein[J]. J Biol Chem, 1975, 250: 4007-4021.
[5] Gygi SP, Corthals GL, Zhang YN, et al. Evaluation of two- dimensional gel electrophoresis-based proteome analysis technology[J]. Proc Natl Acad Sci USA, 2000, 97(17): 9390.
[6] Molloy MP.Two-dimensional electrophoresis of membrane proteins using immobilized pH gradients[J]. Anal Biochem, 2000, 280: 1.
[7] Zuo X, Speicher DW.A method for global analysis of complex proteomes using sample prefraction by solution iso-electrofocusing prior to two-dimensional electrophoresis[J]. Anal Biochem, 2000, 284: 266.
[8] Chen Rong-Zhi, Weng Qing-Mei, Huang Zhen, et al. Analysis of Resistance-related Proteins in Rice Against Brown Planthopper by Two-dimensional Electrophoresis[J]. Acta Botanica Sinica, 2002, 44(4): 427-432.
[9] 逯斌,林兵. 一种改良的植物蛋白质双向电泳方法[J]. 生物化学与生物物理进展,1989,16(6):480-481.
[10] 魏磊,丁毅,胡耀军,等. 紫稻细胞雄性不育系叶片全蛋白质双向电泳分析[J]. 遗传学报,2002,29(8): 696-699.
[11] 陈伟,黄春梅,吕柳新. 顽拗植物荔枝蛋白质双向电泳的改良方法[J]. 福建农业大学学报,1999,6(2): 142-145.
[12] 钟伯雄. 水稻蛋白质双向电泳分析新方法[J]. 浙江农业大学报,1997(2): 128-132.
[13] Chen Rong-Zhi,Weng Qing-Mei,Huang Zhen.Analysis of Resistance-related Proteins in Rice Against Brown Planthopper by Two-dimensional Electrophoresis[J]. Acta Botanica Sinica,2002,41(4): 427-432.
[14] 段海峰,钱令嘉,应万淘,等. 应激心肌细胞蛋白质组双向凝胶电泳分析[J]. 中国生物化学与分子生物学报, 2002,18(2): 234-239.
[15] 汪家政,范明. 蛋白质技术手册[M]. 北京: 科学出版社,2002
(第一版),51-54.
[16] 郭尧君. 蛋白质电泳实验技术[M],北京: 科学出版社,2001
(第一版),141-144.

The Improvement of Isolation and The Method of Two-Dimensional Electrophoresis of Protein from Tea Plant

PDF (PC)

Knowledge

Cited

Abstract

Cite this article

share this article

References

Related Articles 15

Metrics

Comments

Recommended 0

[1]	TANG Haikun, ZHANG Lanjun, ZHANG Panpan, LIU Benying. Research Progress on Chemical Constituents and Biological Activities of Alkaloids in Tea [J]. Journal of Tea Science, 2025, 45(5): 727-741.
[2]	LI Guinan, YANG Ni, LUO Wei, ZHANG Jiaqi, HU Zhihang, XIONG Aisheng, HAO Jiannan, ZHUANG Jing. Identification of CsDET2 and Its Response Analysis to Photoperiod and Abiotic Stress in Camellia sinensis [J]. Journal of Tea Science, 2025, 45(5): 742-756.
[3]	FAN Yangen, XIAO Yue, MENG Fanyue, LIU Wenjie, ZHANG Ying, SUN Ping, ZHANG Lixia, REN Lijun. Cloning and Functional Analysis of the Anthocyanin Synthase Gene CsANS1 in the Purple Bud Tea Variety ‘Zijuan' [J]. Journal of Tea Science, 2025, 45(5): 757-769.
[4]	TU Yiyi, ZHANG You, XU Ting, CHEN Junjie, WANG Yuchun, LÜ Wuyun. Loop-mediated Isothermal Amplification-based Detection of Colletotrichum camelliae [J]. Journal of Tea Science, 2025, 45(5): 770-782.
[5]	JIANG Li, LI Duojiao, HU Xinrong, SHEN Yingzi, ZHENG Zhaisheng, WENG Xiaoxing, LIU Shujing, BIAN Xiaodong, YUAN Ming'an, CHEN Xuan. Effects of Different Cultivation Patterns on Physiological and Biochemcial Characteristics of New Shoots in Seed-Leaf Dual-Purpose Tea Plants [J]. Journal of Tea Science, 2025, 45(5): 783-794.
[6]	WANG Kairong, ZHANG Longjie, LIANG Yuerong, LI Xiaoxiang, ZHENG Xinqiang. Identification and Classification of Tea Leaf Color and Establishment of A Tea Leaf Color System [J]. Journal of Tea Science, 2025, 45(5): 795-807.
[7]	LI Jing, HU Xinlong, TANG Huishan, GUO Jinling, HU Guangcan, FENG Depin, QIU Fangfang, WANG Mingle. Integrated Sensory Evaluation and Metabolomics Analysis of the Quality Characteristics of Yihong Black Tea with Different Levels of Tenderness [J]. Journal of Tea Science, 2025, 45(5): 808-820.
[8]	GUO Yu, XIAO Liuyu, DU Qiuyi, TIAN Ye, HAN Yu. Study on Comprehensive Optimization of the Extraction Process and Emulsion Loading System of Water-extracted Polysaccharides from Qingzhuan Tea and Alkali-extracted Polysaccharides from Tea Residue [J]. Journal of Tea Science, 2025, 45(5): 821-840.
[9]	SU Lin, HUANG Zihao, SUN Dan, CHEN Jinhua, ZHENG Yajie, LU Ying. Study on the Hypoglycemic Effects of Major Compounds in White Tea Based on Network Pharmacology and Zebrafish Model [J]. Journal of Tea Science, 2025, 45(5): 841-851.
[10]	WANG Yonghui, WANG Duofeng, LI Xuemin, SHI Tianbin, WU Lidong, LIU Zaiguo, ZHANG Guangzhong, ZHAO Fengyun. Study on the Physicochemical Components and in Vitro Antioxidant Differences of Green Tea from Longnan, Gansu and Jinhua, Zhejiang [J]. Journal of Tea Science, 2025, 45(5): 852-864.
[11]	CHEN Junrui, HU Junming, SHI Yuanzhi, WEI Xianghua, SONG Chuankui, ZHANG Junhui, ZHENG Fuhai, SUO Guangli. The Effects of Biochar-based Fertilizer on the Physical Stability of Organic Carbon in Soil Aggregates of Tea Gardens [J]. Journal of Tea Science, 2025, 45(5): 865-878.
[12]	LI Bing, ZHU Yong, XIA Chenglong, LI Feilong, CAI Zhenyang, WU Hao. Lightweight Online Sorting Method of Milled Tea Based on Improved YOLOv5s [J]. Journal of Tea Science, 2025, 45(5): 879-897.
[13]	MENG Chao, LIANG Tao, ZHANG Xia, WANG Wanhong, DONG Huanglin, LI Ming. Research on Tea Light Complementary Mode Based on Tea Planting and Photovoltaic Power Generation [J]. Journal of Tea Science, 2025, 45(5): 898-908.
[14]	ZHOU Yide, CHEN Jialin, WU Junmei, ZHAO Hongbo, SUN Binmei, LIU Shaoqun, ZHENG Peng. Nitrogen Metabolism Genes in Tea Plant: Research Progress on the Environmental Stress Adaptation Mechanism and Breeding Application [J]. Journal of Tea Science, 2025, 45(4): 545-558.
[15]	SUN Mengzhen, HU Zhihang, YANG Kaixin, ZHANG Jiaqi, ZHANG Nan, XIONG Aisheng, LIU Hui, ZHUANG Jing. Identification of Circadian Clock CsLUX Gene and Its Effects on Photosynthetic Characteristics in Tea Plants [J]. Journal of Tea Science, 2025, 45(4): 559-570.