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Journal of Tea Science ›› 2003, Vol. 23 ›› Issue (2): 105-109.doi: 10.13305/j.cnki.jts.2003.02.004

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Damage of Oxidant-stress and Effect of Epigallocatechin-3-gallate on Vero Cells in Vitro

HU Xiu-fang1, CHAI Li-hong, YANG Xian-Qiang3   

  1. 1. College of Life Science, Zhejiang University;
    2. Institute of Nuclear Agricultural Sciences, Zhejiang University;
    3. Department of Tea Science, Zhejiang University, Hangzhou 310029, China
  • Received:2002-11-24 Revised:2003-05-26 Online:2003-11-25 Published:2019-09-16

Abstract: This study was conducted to identify the protection of antioxidant--epigallocatechin-3-gallate(EGCG) to the renal damage by oxidant-stress. H2O2 and Cr6+ were used to induce apoptosis of renal cells-----Vero cells in vitro. With these models of cell apoptosis, the effect of EGCG on apoptosis was investigated through Staining and DNA electrophoresis. As the results, H2O2 and Cr6+ decreased the viability of Vero cells in a dose-dependent manner, and the IC50 was 175.6 and 9.8 mg·L- respectively. Both 50 mg·L-1 H2O2 and 400 mg·L-/2h Cr6+ caused the apoptosis of Vero cells. 40 mg·L- EGCG markedly inhibited the apoptosis of Vero cells, and the effect was the same as that of Vc and EDTA in combination, indicating the simultaneous effect of antioxidation and chelating. So, EGCG protected Vero cells from damage through both ROS scavenging and ion-chelating, and this kind of effect is valuable to kidney usually exposed to ROS in clinic.

Key words: Biomedicine, renal cells, Oxidant-stress, Apoptosis, EGCG

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