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Journal of Tea Science ›› 2005, Vol. 25 ›› Issue (1): 7-15.doi: 10.13305/j.cnki.jts.2005.01.002

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Construction of AFLP Molecular Markers Linkage Map in Tea Plant

HUANG Jian-an1, 2, LI Jia-xian3, HUANG Yi-huan2*, LUO Jun-wu2*,GONG Zhi-hua2, LIU Zhong-hua1, 2   

  1. 1.Key Laboratory of Tea Science of Ministry of Education, ChangSha 410128, China;
    2.Tea Science Department, Hunan Agricultural University, ChangSha 410128, China;
    3.Tea Research Institute, GuangDong Academy of Agricultural Sciences, YingDe 513042, China
  • Online:2005-03-25 Published:2019-09-16

Abstract: Based on an improved method of AFLP, AFLP markers were employed for construction of a linkage map used a set of 69 F1 progenies of Qi Men No.4×Chao An Da Wu Ye as a mapping population. In this work, all together 1925 bands were obtained by 22 pairs of primers and 87.5 bands each primer pair on average, a total of 485 (25.19%) polymorphic AFLP markers were detected. The 356 (73.40%) of polymorphic markers with Mendelian segregation ratio (P=0.01) were obtained. 247(69.38%)of the 356 loci were 1:1 segregation, 109(30.62%)of the 356 loci were 3:1 segregation. Furthermore, the AFLP molecular markers linkage maps of Qi Men No.4 and Chao An Da Wu Ye were constructed respectively by using Mapmaker/Exp (version3.0). The map of female parent included 17 linkage groups and located 208 markers, covered total map length of 2457.7 cM, and average distance between markers was 11.9 cM. The map of male parent included 16 linkage groups and located 200 markers,covered total map length of 2545.3 cM, and average distance between markers was 12.8 cM.

Key words: Tea plant, Molecular markers linkage map, AFLP

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